Single Nucleotide Polymorphism Of Growth Differentiate Factor5and Lumbar Degenerative Disease

Part: Association between the Growth Differentiate Factor5Polymorphism and the Lumbar Disc DegenerationObjective: To investigate the association between single nucleotide polymorphisms(SNP) of growth differentiation factor5(GDF5), rs143383, and lumbar disc degeneration(LDD).Methods: Genome DNA was extracted from peripheral blood leucocytes in272LDDgroups and311control subjects, and SNP rs143383was analyzed by Taqman probemethod. Odds ratios (Odds ratio, OR) and its95%confidence interval (confidence interval,CI) values was used to reflect its effect, and multivariate logistics regression analysis wasused to calculate the adjusted OR.Results: Genotypes were found in Hardy-Weinberg equilibrium both in LDD groupand control group (P>0.05). In the dominant model (TT vs. TC/CC), SNP rs143383lociassociated with the occurrence of LDD (P=0.041; OR=1.42,95%CI:1.01-1.98); whilein the recessive model (TT/TC vs. CC), rs143383SNP also associated with LDDoccurrence (P=0.031; OR=0.43,95%CI:0.19-0.94). Subgroup analysis showed infemale patients SNP rs143383was also significantly correlated with LDD (P=0.023; OR=1.83,95%CI=1.20-2.79). After multivariate analysis, the adjusted OR was1.365, with95%CI1.079-2.569.Conclusions: GDF5rs143383SNP play an important role in the development andprogression of LDD. T allele exist may increase the incidence of LDD. This association isparticularly evident in women. Part: Association of the Growth Differentiate Factor5Polymorphism and the Extent of Lumbar Disc DegenerationObjective: To investigate the association between single nucleotide polymorphisms(SNP) of growth differentiation factor5(GDF5), rs143383, and the severity of lumbar discdegeneration (LDD).Methods: SNP analysis using the first part of research results. Pfirrmann gradingsystem respectively on two groups of people (LDD group, control group) to grading theseverity of intervertebral disc degeneration. Using Thompson pathological grading systemfor surgery in the LDD group for grading the severity of intervertebral disc degeneration.Results: Genotypes were found in Hardy-Weinberg equilibrium (P>0.05). In thedominant model (TT vs. TC+CC), genotype frequencies difference were statisticallysignificant in different magnetic resonance Pfirrmann grading (χ2=15.284, p=0.004). Inthe recessive model (TT+TC vs. CC), genotype frequencies difference were alsostatistically significant in different Pfirrmann grading (χ2=10.035, p=0.040). In thedominant model (χ2=4.411, p=0.220) and recessive model (χ2=3.670, p=0.299),Thompson classification level of different genotype distribution in frequencydifference were no statistical significance.Conclusions: Rs143383called GDF5were gene loci single nucleotidepolymorphisms and the han people of lumbar degenerative severity are closely related. Part: Correlation Studies between the Growth DifferentiateFactor5Polymorphism and the Degenerative Lumbar DiseasePostoperative EfficacyObjective: To investigate the relevance between growth differentiation factor5geners143383single nucleotide polymorphisms (SNPS) and degenerative disease of lumbarsurgery effect. Methods: Selecting LDD group in102cases of surgical treatment of patients. SNPanalysis using the first part of research results.Postoperative follow-up system,using theOswestry disability index questionnaire (Oswestry disability index, ODI) to evaluate thepostoperative dysfunction; By using Visual Analogue Scale (Visual Analogue Scale, VAS)to assess postoperative pain; According to the results of the clinical manifestations ofcombined imaging examination to assess postoperative relapse.Results: Genotypes were found in Hardy-Weinberg equilibrium (P>0.05). Accordingto the dominant model,61patients carry TT genotype, while41patients carry TC+CCgenotype. The follow-up rate was90.2%. The ODI and VAS at6month postoperativelydecreased significantly compared with preoperative ODI and VAS, but the differencebetween the two genotype groups was not statistically significant (P>0.05). At1year and2years postoperatively, the difference between two genotype groups were still notstatistically significant (P>0.05). But the differences between the two genotype groupswere statistically significant at3years (P <0.05). After six months, the recurrence ratebetween the two genotypes group comparison differences had no statistical significance(P>0.05);At1year and2years postoperatively, the recurrence difference between twogenotype groups were still not statistically significant (P>0.05). But the differencesbetween the two genotype groups were statistically significant at3years (P <0.05).Subgroup analysis showed that this effect is more pronounced in female patients.Conclusions: Surgery can effectively improve the patient’s symptoms, a gene calledGDF5were rs143383site postoperatively in patients with single nucleotidepolymorphisms affect curative effect, carry the T gene in patients with long-term prognosisis relatively poor, especially the phenomenon of female patients. Part: Correlation Studies between GDF5Single NucleotidePolymorphisms and the Lumbocrural Pain Incidence ofAsymptomatic Lumbar Degeneration Crowd Objective: To investigate the relationship between growth differentiation factor5gene rs143383single nucleotide polymorphisms and the incidence of lumbocruralpainasymptomatic lumbar disc degeneration (LDD).Methods: Selecting LDD group in108cases of asymptomatic (Pfirrmann-level).SNP analysis using the first part of research results.Postoperative follow-up system,Kaplan-Meier curves were used to depict the time of lower back pain symptoms appear.Log-rank test and multivariate Cox proportional hazard model were used to analysis in thedominant model (TT vs. TC+CC). Effect values were expressed in HR (Hazard Ratio)and95%CI (confidence intervals).Results: Genotypes were found in Hardy-Weinberg equilibrium (P>0.05). Accordingto the dominant model,66patients carry TT genotype, while42patients carry TC+CCgenotype. Follow-up period without1case death, lost to8cases, follow-up rate was92.6%.A total of15cases (13.9%) occurred during follow-up of lower back pain symptoms in108asymptomatic LDD patients. The incidences were19.1%(13/66) and4.8%(2/42) in TTgenotype group and TC+CC genotype group, respectively. Log-rank test showed that TC+CC genotype group has lower the incidence of symptoms compared with TT genotypehigh (χ2=4.79, p=0.029). The HR was4.905, with95%CI1.009-46.659. Subgroupanalysis showed in the female subgroup, the incidences were32.0%(8/25) and5.0%(1/20)in TT genotype group and TC+CC genotype group, respectively. Log-rank test showedthat TC+CC genotype group has lower the incidence of symptoms compared with TTgenotype high (χ2=5.063, p=0.024). The HR was8.944, with95%CI1.001-417.100.Conclusions: GDF5rs143383SNP is has certain effect on the incidence ofasymptomatic lumbar degeneration crowd lumbocrural pain, carry the T gene are morelikely to appear lumbocrural pain; This phenomenon is particularly women.