1.Long-term Effect Of Early-life Supplementation With Probiotics On Preventing Atopic Dermatitis 2.Differential Expression Of Proteins Associated With The HairFollicle Cycle-Proteomics And Bioinformatics Analyses

Background:The prevalence of allergic diseases has increased over the past few decades. Atopic dermatitis (AD) is a chronic, pruritic and inflammatory skin disorder, which normally presents in early childhood and persists into adult in 60% of patients. All available treatments nowadays cannot cure but alleviate the symptoms of AD. Developing a new method to prevent AD is of great importance. Administration of probiotics in early life may be an effective method to prevent AD, but very little is known about its long-time preventive effect. Objective:To systematically evaluate the long-term effect of probiotics on preventing atopic dermatitis. Methods: Databases including PubMed、 Web of Science、Cochrane Library、EMBASE、CBM and CNKI were searched from inception to July 31,2014, so as to collect the randomized controlled trials (RCTs) about probiotics on preventing atopic dermatitis as long as at least 5 years. Two reviewers independently screened articles according to the inclusion and exclusion criteria, extracted data, evaluated quality and checked. After that, the meta-analysis was conducted using RevMan 5.2 software. Result:Six trials and a total of 1955 patients were included in this meta-analysis. Risk ratio and 95% confidence intervals were calculated by a fixed effect model (p=0.10, I2=0.46). The combined risk ratio of the meta-analysis comparing probiotics with placebo for investigating the long-term preventive effect of AD was 0.86 (p=0.005, 95% CI 0.77-0.96). There were no significant effects of probiotic consumption (by infants or mothers) on the development of IgE-associated AD in children (p=0.87). Sub-analyses showed that both prenatal and postnatal administration of probiotics would lead to lower cumulative incidence of AD compared with placebo (p=0.002), while no significant effects could be found in children with only postnatal administration (p=0.89). Conclusion:Administration of probiotics during pregnancy and in early life could be an effective tool in the primary prevention of AD during a long-term follow-up period.Objective:To identify differential expression of proteins associated with the hair follicle cycle. Methods:proteomics and bioinformatics analyses were performed to construct comparative protein profiles of mouse skin at specific time points (0,8 and 20 days). Results:Ninety-five differentially expressed protein spots were identified by MALDI-TOF/TOF as 44 proteins, which were found to change during hair follicle cycle transition. Subsequently, three proteins were selected to validate the reliability of expression patterns using western blotting. Cluster analysis revealed three expression patterns, and each pattern correlated with specific cell processes that occur during the hair cycle. Furthermore, bioinformatics analysis indicated that the differentially expressed proteins impacted multiple biological networks, after which detailed functional analyses were performed. Conclusion:the above data may provide insight into the three stages of mouse hair follicle morphogenesis and provide a solid basis for potential therapeutic molecular targets for this hair disease.