Effect Of Hydrogen Sulfide On Gastric Receptive Relaxation

Objective:By the moderating role of hydrogen sulfide in mice gastric receptive relaxation, study of H2S on whether on of the stomach to regulate the function of influence, and to further explore in patients with functional gastrointestinal disease pathogenesis in order to find more effective treatment strategies.Methods:Gastric mucosa of patients with FD. FD in gastric mucosa of Western blot detection of CBS and CSE expression and immunofluorescence; gastric fundus H2S release; CBS+/-mouse fundic muscle tension experiment, mice in the gastric body pressure records (IGP), NANC relaxation of electrical stimulation (EFS) of fundus of stomach muscle strip, the H2S signaling pathway on food intake.Results:Western blot was used to confirm the expression of cystathionine--synthetase and cystathionine--lyase. Immunohistochemical studies showed that CBS was expressed in the cytoplasm of the neurons in the nerve plexus of the stomach.In addition, the muscle tissue bundles showed a clear immune response to CSE. A large number of H2S was detected in the stomach of mice, and the gastric fundus had the ability to synthesize hydrogen sulfide from L-cysteine. Mice stomach bottom or stomach muscle strips showed spontaneous contraction, sodium sulfide in the stomach or stomach can cause relaxation; L-cysteine, also induced inhibition of mouse gastric fundus contraction activity. On NANC nerve stimulation reaction of tip: after addition of L-cysteine in the bath in gastric fundus smooth muscle of EFS relaxation in response to increased significantly, however, in the bath with AOAA, the relaxation amplitude of EFS was greatly reduced. The expression of CBS protein increased after 5 min after eating, reached a peak at 10 min, and decreased to normal after 20 min of feeding. However, another H2S generating enzyme CSE did not change after eating. The production of hydrogen sulfide in mice after eating was also increased, but this change was not in the stomach. Food intake and body weight were significantly reduced after injection of AOAA, however, whether L-cysteine or NaHS, the food intake and body weight change were not affected. CSE inhibitor PAG or CBS agonist SAM had no effect on food intake and body weight change, which was consistent with the gastric compliance test.Conclusion:In this research, we use CBS+/-mice of H2S stomach regulation, found that mice gastric bottom express H2S generating enzymes (CBS and CSE), to produce hydrogen sulfide can be detected. Sodium hydrogen donor or L-cysteine, which causes the stomach or stomach to relax. Gastric compliance increased significantly in the presence of L- cysteine. In contrast, AOAA, an inhibitor of CBS, can inhibit gastric compliance. So, CBS+/-mice showed low gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliance. L-cysteine increased the non adrenergic non cholinergic (NANC) muscle relaxation, but decreased the amplitude of EFS by AOAA. It is worth noting that the expression level of CBS increased after feeding, but the CSE protein did not appear to be such a change. Moreover, the production of H2S in the stomach of mice increased after feeding. In addition, AOAA reduced food intake and body weight in mice. In addition, patients with functional dyspepsia (FD) found H2S metabolic abnormalities. Therefore, endogenous H2S, a novel gas signaling molecule, is involved in the regulation of the stomach.