The Clinical And Experimental Study Of Specific Immunotherapy Combined With Traditional Chinese Medicine On Pediatric Asthma

Objective1.To investigate the main allergens and their distribution patterns in children being under traditional Chinese therapy in Guangzhou to provide evidence for the prevention and treatment with traditional Chinese medicine of allergic diseases in children.2. By learning with Professor You Jia Xu,Organizing124 cases of pediatric asthma systematic to analyze its drug law, to investigate the "children of Deficiency" theoretical connotation and its clinical significance.3. Prospective, randomized study to evaluate the efficacy and side effect of specific immunotherapy combined with traditional Chinese medicine on pediatric asthma.4. To observe the effects of ShengQiZhuangYang medicinal extract combined with specific immunotherapy on airway inflammation of mouse with bronchial asthma, and to explore itsmechanism in treating asthma.Methods1. Skin prick test was performed by using liquid with 13 standardized allergens (ALK-Abello) on 473 children who were treated by traditional Chinese medicine in Guangdong Province Hospital of Traditional Chinese Medicine between April 2008 and February 2011.2. With TCM inheritance assist system software, select Pro Xu’s 124 cases of pediatric asthma at the Guangdong Provincial Hospital pediatric department, prescription of using Chinese herbs frequency, meridian, four qiand five flavors, Chinese medicine herbs combinations, herbs paring and summarize law of consultion.3. A randomized, controlled method was conducted, children with asthma were randomly divided into treatment group (specific immunotherapy+ traditional Chinese medicine) and control group (specific immunotherapy group), Observation period is 50 weeks, Symptom scores, PEF, PEF mutation rate, the incidence of adverse reactions were recorded in week 4、9、13、17、21、 25、29、35、41、47、50.4. TwentyBALB/c mice were randomly divided into blank control group, asthma group, SIT groupand SIT+ medicinal extract group. Asthma was induced by intraperitoneal injection of ovalbumin (OVA) andforced inhalation of atomized OVA. Thenumbers of the total inflammation cells and esoinophils(EOS) in BALF were counted by alveolar wash. IL-4、IFN-γ in BALF and IgE in blood were analysed by ELISA. Pathological changes of the lung tissues were observed by HE straining.Results1. The positive rate of skin prick test was 76.32%(361/473), and the top three prevalent allergen were Dermatophagoides farina (71.67%) Dermatophagoides pteronyssinus (68.71%) and Blomia tropicalis (37.21%).In the positive cases, Only 4.99% cases had a single allergen while 95.01% children had two or more allergens. There was no statistically significant difference between males and females (P>0.05). The positive rate of school-age children was higher than preschool children.The positive rate of school-age children and adolescence had no differences.2. Professor Xu Jia’sprescription drugs treatment of pediatric asthma mainly by the tonic, relieving drugs, Qi medicine, digestion drug and astringent components.124 prescription drugsare radix sileris, Atractylodes, licorice, orange peel, Schisandra, fried rice shoots, betel nuts, Shidi, heterophylla and bergamot like. Professor Xu Jia treat of pediatric asthmamainly with warm drug, followed by the level of the goods, less cold, cold drugs, few heat.3. Randomized controlled studies have found that the incidence of adverse reactions between the two groups have statistical significance, in terms of PEF PEF variation rate, began to appear in the 17 weeks treatment group improved significantly, while the control group only a similar change in 21 weeks.4. After the therapy, the lung inflammation was reduced, and the IgE level in blood was lowered, especially in the SIT+medicinal extract group, and the numbers of total cells and EOS decreased. IFN-y level was raised and IL-4 level was lowered in BALF, whice has no differences between the SIT group with the SIT+ medicinal extract group.Conclusion1. The main allergens for allergic diseases are Dermatophagoides farina and Dermatophagoides pteronyssinus in Guangzhou. Single allergen is rare. The positive rate has no statistically significant difference between males and females. The positive rate increases with age from preschool to school-age.2. Professor xu follow the theory of Children’s constitution is deficiency cold when treating the pediatric asthma; Commonly usingtonify deficiency、Qi medicine and digestion drug, attaches great importance to the diet life regulating guide.3. Standardized specific immunetherapy combined with traditional Chinese medicine, can shorten the work time, reduce the systemic adverse reactions, is of great significance to improve the therapeutic efficacy of pediatric allergic asthma.4. ShengQiZhuangYang medicinal extract combined with specific immunotherapy can reduce the inflammation of lung and lower IgE level in blood...