Study On The Processing Technics And Quality Standard Of Typha Angustifolia L.

Carbonized traditional Chinese medicine is unique in the world, which can be used totreat many kinds of haemorrhage symptoms and other diseases. Pollen Typhae Carbonisatusis the product of Pollen Typhae and has been used for a long time. The clinical practice hasproved that Pollen Typhae Carbonisatus had significant effects in hemostasis. It can be usedfor the treatment of blood vomiting, leaking and other haemorrhage disorders.Nine sections are included in this dissertation with the purpose of research on thePollen Typhae.In the first part, there is a documental review of the scientific research on PollenTyphae including the history of the craft and the primary intention of thepredecessors,chemical constituents, pharmacological effects, clinical application andprocessing case. We summarized their experiences and clinical comprehensions, whichprovided effective experiences to our research.Based on the theory of traditional Chinese medicine, we select the effective part anddemonstrated the effective part of Pollen Typhae, Pollen Typhae Parch and Pollen TyphaeCarbonisatus through the chemical separation and pharmacodynamical screening.Through the evaluation of the quality and quantity of the effective part, we selected theoptimum method by the orthogonal designed experiment: Pollen Typhae Parch was roasted12 minutes at 150℃and Pollen Typhae Carbonisatus was roasted 8 minutes at 210℃. Wealso formulated the standard operation practice （SOP） scientifically and precisely.We established the method based on the systematic research on the herbal piecesdescription, identification, qualitative and quantitative analysis of Pollen Typhae, PollenTyphae Parchand Pollen Typhae Carbonisatus. We formulated the appropriate methods ofthe quality control for them respectively.We inspected the stability and the accelerative stability 3 batches of samples. We alsocompared the influence of the different wrapping materials on the herbal pieces, includingconventional plastic, paper and aluminum foil.We studied the extractions from pollen typhae. Twelve components were isolated and identified by spectrum technology such as IR、¹H-NMR, ¹³C-NMR、HSQC、HMBC、¹H-¹H-COSY and ESI-MS. The structure of them were icosanoic acid,stearic acid,β-sitosterol, vanillic acid, stearic acid propanetriol ester, quercetin, naringenin,2-hydroxy-N-（（4Z,8Z）-3-hydroxy-1-（3,4,5-trihydroxy-6-（hydroxy methyl）-tetrahydro-2H-pyran-2-yloxy）octadeca-4,8-dien-2-yl） tetradecanamide, pyrimidine-2,4（1H,3H）-dione,β-sitosteryl-3-O-glucopyranoside, isorhamnetin-3-O-neohesperidoside,typhaneoside. Among them, the compoundsâ…¤,â…§, andâ…¨were isolated from PollenTyphae for the first time. We also compared the tannic contents of Pollen Typhae, PollenTyphae Parch and Pollen Typhae carbonisatus.We performed preliminary research on the fingerprint pictures of Pollen Typhae and itsprocessing herbal pieces. Based on the fingerprint pictures of Pollen Typhae, Pollen TyphaeCarbonisatus and Pollen Typhae Parch by HPLC. The chromatographic conditions were asfollowed: Waters X-Terra C₁₈（4.6mm×25cm） with the gradient elution solvent systemmethylcyanid-0.05%trifluoroacetic acid under the detected wavelength of 254 nm for PollenTyphae and Pollen Typhae Parch, 360nm for Pollen Typhae Carbonisatus,flow rate of1.0ml/min. Based on the work mentioned above, we established the standard fingerprintpictures of Pollen Typhae, Pollen Typhae Parch and Pollen Typhae Carbonisatus.