Research On The Influence Of Vibration Training On Body Composition In Postmenopausal Females And The Association To Related Gene Polymorphism

Ⅰ Purpose and Significance of ResearchThe body composition of postmenopausal females often changed with aging, such as body fat increasing, lean mass and bone mass decreasing. As a new form of exercise, vibration training has been paid extensive close attention. However, the influence of vibration training on body composition and individual difference of training effects have not been studied systematically. In this study, through the regular vibration training in postmenopausal females, the effects of vibration training on body composition were explored, and then the association between related gene polymorphism and the vibration-induced body composition changes were investigated, sensitive molecular markers were screened. In order to clarify the molecular regulation mechanism investigated the biological function of polymorphism loci. The results may provide experimental evidence for develop the personalized vibration training prescription.Ⅱ Contents and Experimental Results of ResearchThe first part is to study the influence of vibration training on body fat and lean mass and the association between the influence effects and MSTN, ADPN gene polymorphism. Through 3 months vibration training, the FM% significantly decreased, meanwhile, LM% increased. The association analysis between vibration-induced body composition changes and MSTN, ADPN gene polymorphism revealed that the different genotypes of MSTN gene (rs3791783, rs7570532, rs3791782) and ADPN gene rs6773957 were associated with the effects of vibration training on body composition. Through 15 months vibration training in rs3791783/AG+GG subjects, there were no significant changes in body fat and lean mass.The second part is to study the influence of vibration training on BMD and the association between the influence effects and OPG-RANK-RANKL gene polymorphism. Through 3 months vibration training, the BMD remained unchanged, and there were no significant association between vibration-induced BMD changes and OPG-RANK-RANKL gene polymorphism. Through 15 months vibration training, we found that the vibration training significantly inhibited bone loss in L2-4 with aging. The association analysis between 15 months vibration-induced BMD changes and OPG-RANK-RANKL gene polymorphism revealed that different genotypes of RANKL gene (rs3742257 and rs9525641) were associated with the effects of vibration training on BMD.The third part is to study the biological activity of MSTN polymorphism loci. The second intron gene sequences of MSTN contained Loci (rs3791783 and rs7570532) were synthesized and site-directed mutagenesis, connected to pGL3-promoter vector to construct recombinant plasmids respectively, and then the reporter gene expression after 48 h C2C12 cells transfected with recombinant plasmids were detected. The results revealed that there were significant differences of dual-luciferase gene expression levels in cells in different genotypes of the same site.Ⅲ Conclusionsⅰ Three-month vibration training reduced percent of fat mass and increased percent of lean mass in postmenopausal females, but it had no effect on BMD. However, 15-months vibration training significantly inhibited the bone loss with age in postmenopausal females.ⅱ MSTN gene (rs3791783, rs7570532, rs3791782) and ADPN gene rs6773957 might be used as sensitive molecular marker of the effects of vibration training on body fat and lean mass. RANKL gene (rs3742257 and rs9525641) might be used as sensitive molecular marker of the effects of vibration training on BMD.ⅲ rs3791783 and rs7570532 polymorphic loci affected downstream gene expression activity, may be an important regulatory factors of the vibration training effects on body composition.