The Study Of The Impact Of Isolated Teratozoospermic Men On Outcome Of Human Assisted Reproduction Technique And The Chromosomal Anomaly Of Their Sperms And Embryos

Objective:The aim of this study is to compare conventional IVF with ICSI in males with isolated teratozoospermia as defined by the WHO-5manual.Methods:A retrospective study was performed in included2178infertile couples undergoing IVF/ICSI treatments in the reproductive centre of Jiangxi Hospital for Women and Children in September2010-March2012. Included cycles were divided into4groups:group1(n=1971):normal sperm morphology with conventional insemination; group2(n=153):isolated teratozoospermia with conventional insemination; group3(n=27):normal sperm morphology with ICSI; and group4(n=27):isolated teratozoospermia with ICSI. Fertilization, totally fertilization failure, embryo quality, blastocyst formation rate and pregnancy rate were studied between groups. After that, we did the multi factor analysis to figure out the factors that may related with fertilization.Results:The fertilization rate of the group2(52.2±26.6%) was lower than group1(58.0±23.6%, p<0.05).And the difference of fertilization rates between other groups was not statistically significant (p>0.05). Another critical observation was that the totally fertilization failure rate of the group2(11.1%) was significantly higher than the one of the group1(5.4%, p<0.05).No statistically significant difference was noted in the other fertility indices (e.g. rates of high quality embryos, blastocyst formation and pregnancy) between groups.(Table1). Besides, by multiple variables analysis we found that many factors including percentage of morphologically normal sperm were statistically correlated with fertilization rate and totally fertilization failure in conventional IVF cycles. After that, it was also shown that several factors including the insemination method were correlated with fertilization rate in cases with isolated teratozoospermia.Conclusion:In conventional IVF cycles a significantly lower fertilization rate and higher total fertilization failure rate were observed in couples with isolated teratozoospermia as compared to couples with a normal semen profile. Furthermore, when ICSI was used to treat these teratozoospermic couples, improvement in fertilization was noted. Objective:The aim of this study was to evaluate the effect of isolated teratozoospermia on the clinical pregnancy rate of AIH on a large population.Methods:A retrospective study was performed in infertile couples undergoing AIH treatments in the reproductive centre of Jiangxi Hospital for Women and Children in September2010-June2012. Included cycles were divided into3groups:Isolated teratozoospermia group:<4%normal morphologic forms and normal sperm concentration and motility; Normozoospermia group:normal sperm concentration, motility and>4%normal morphologic forms; other male factor infertility group: asthenozoospermia or oligozoospermia with or without teratozoospermia. The clinical pregnancy rates of the first AIH attempt and the cumulative clinical pregnancy rates between the3groups were compared.Results:The three groups were similar with regard to female age, female infertility factors, and female infertile years, etc. The overall clinical pregnancy rate of the first AIH attempt and the cumulative clinical pregnancy rate were12.3%(31/253) and18.9%(48/253). The clinical pregnancy rates of the first IUI attempt between the3groups were not significantly different. The cumulative clinical pregnancy rates of the normozoospermia group (25.6%) was significantly higher than the ones of the other2groups (12.5%of the teratozoospermia group and10.9%of the other male factor infertility group)(p<0.05). The cumulative clinical pregnancy rates of the isolated teratozoospermia group and other male factor infertility group were not significantly different.Conclusion:This study documented that the cumulative clinical pregnancy rates was significantly and similarly reduced in couples with isolated teratozoospermia as in couples with other sperm defects, when compared to couples with normozoospermia. Because the clinical pregnancy rate per embryos transfer cycle of IVF/ICSI in the reproductive centre of Jiangxi Hospital for Women and Children (50%) is higher than the cumulative clinical pregnancy rate of AIH in our center (18.9%), these patients should be counseled about the possibility to proceed more rapidly to IVF or ICSI. Objective:To explore the sperm chromosome abnormalities with teratozoospermic men.Methods:Sperms obtained from isolated teratozoospermic men (n=18) and control men with normal fertility (n=5) were analyzed using fluorescent in situ hybridization (FISH) to detect chromosome abnormalities of chromosomes18, X and Y.Results:Totally58178spermatozoa were counted from the teratozoospermia group and16369spermatozoa were counted from the control, with the hybridization rates of97.5%and98.3%, respectively. The major types of chromosome abnormalities were disomy (YY18, XX18, XY18, Y1818and X1818) and diploidy (1818XX,1818YY,1818XY). In the teratozoospermic group and the control group, the disomy rates of18chromosome were0.29±0.16%and0.03±0.02%, the disomy rates of sex chromosome were0.65±0.24%and0.05±0.02%, the diploidy rates were0.14±0.12%and0.04±0.03%, respectively. All the differences between the2groups were significant (P<0.05).Conclusion:Sperms of isolated teratozoospermic men had higher rates of18, X and Y chromosome abnormalities than those of the fertile controls. Objective:To compare the rates of numerical chromosome abnormalities in embryos derived from bipronucleated zygotes produced by conventional IVF between isolated teratozoospermic couples and control couples.Methods:Embryos donated for research were fully biopsied, and their cells were analyzed by fluorescence in situ hybridization with specific probes for chromosomes X, Y and18.Results:There was no statistical difference in chromosome normality rates of discarded embryos between isolated teratozoospermic couples group(40.7%,24/59) and the control group (46.0%,23/50), p>0.05.Conclusion:In conventional IVF cycles, the risk of embryo chromosomal abnormality did not increase in isolated teratozoospermic couples.