Effects Of Curcumin On Rats With Type 2 Diabetes And 3T3-L1 Preadipocytes PTP1B Pathway

Objective:Diabetes mellitus(DM) is a chronic, metabolic diseases, which cause serious harm to human health. The prevalence of obesity has increased dramatically, it has been a problem about the healthy of world people, so it has become a focus of medicine scientific researcher to find an effect drug to prevent and control obesity. The purpose of our research is to observe the effect on the treatment of curcumin to T2DM.,And to find the mechanism of curcumin.Method1.After induction of differentiation on3T3-L1preadipocytes, we observeed the effect of curcumin on differentiation and glucose uptake, and detect the effect of curcumin on adipocyte’s PPARy, C/EBPamRNA, SREBP-1expression by Real-time PCR..2. After induction of differentiation on3T3-L1preadipocytes, we observeed the effect of curcumin on differentiation and glucose uptake, and detected the effect of curcumin on adipocyte’s visfatin, resistin and adiponectinexpression by Real-time PCR.3. The T2DM rats were divided into3groups. The changes of weight, blood glucose, blood lipid,insulin, HOMA-IR,ISI TNF-α、FFA were detected, respectively.4. The T2DM rats were divided into3groups. The changes of Liver weight, liver index, liver pathological, PTP1B. IRS-2、IRS-1mRNA expression and PTP1B, IRS-2protein expression were detected, respectively.5. The T2DM rats were divided into3groups. The changes of muscle PTP1B, GLUT4, IRS-1mRNA expression and GLUT4and PTP1B protein expression were detected, respectively.RESLUTS1. Curcumin can increase glucose uptake and utilization of fat cells, and promote the key factor of adipocyte differentiation---PPARγ and C/EBPamRNA’s expression.Its effect is similar as PPARC agonist pioglitazone.2. Curcumin can inhibit visfatin, resistin mRNA expression, and enhance expression of adiponectin mRNA. promote adipocyte proliferation at low concentrations, inhibit their proliferation at high concentrations, while promoting the differentiation of preadipocytes.3. Curcumin can significantly reduce the glucose and lipid levels, body weight of fat diet combined with low doses of STZ-induced type2diabetic rats. Its also can increase sensitivity to insulin in type2diabetic rats, and improve insulin resistance,.4. Curcumin can significantly reduce type2diabetic rats’FFA TNF-a, thereby increasing sensitivity to insulin in type2diabetic rats, and improveing insulin resistance.5. Curcumin can significantly reduce liver weight and liver in type2diabetic rats index to ease their fatty liver. Its also can significantly reduce PTP1B expression and protein expression in type2diabetic rat liver, and can enhance IRS-1mRNA IRS-2mRNA expression,thereby improving hepatic insulin resistance in type2diabetic rats.6. Curcumin can significantly reduce PTP1B and IRS-1mRNA expression enhance GLUT4mRNA expression and protein expression, thereby improving muscle insulin resistance in type2diabetic rats.Conclusion:The treatment of T2DM with curcumin have a good effect. And the mechanism of that may be the herbs can inhibit the expression of PTP1B pathway.