| 1ObjectveAlzheimer’s disease (AD) is the most common type of clinical dementia, characterized by progressive neurodegenerative, and often accompanied by significant mental and behavioral abnormalities in the certain stage. It seriously affects the life ability and social function of the patient, at the same time also brings heavy burden to family and society, which has become one of the most serious problems facing geriatric medicine today. Comprehensive previous research, there are many hypotheses about AD pathological mechanism, each hypothesis associated with some important biological chemical composition in brain, especially the important biological macromolecules such as protein and enzymes involved in the biochemistry change. At present, β-amyloid(Aβ)pothesis is widely recognized, which points that AP is the key to the pathogenesis of AD, and the chain reaction caused by its toxicity is the basis of pathological changes in AD. Therefore, an important strategy for the treatment of AD is interrupting the vicious cycle caused by Aβ,by means of restraining Aβ secretion and deposition and disturbing the chain reaction caused by its toxicity in the multi-link and multi-path effectively.Professor Li Zhigang based long-term clinical experience combined with previous animal experiments summarized Dredging Governor Meridian and Awakening Mind Method. It is a kind of practical treatment, which can effectively improve the diseases associated with central nervous system by promoting nerve cell regeneration, such as spinal cord injuries and traumatic brain injury. AD has a characteristic of psychology, yet the research about combining the traditional acupuncture and music therapy in the treatment of AD is still rare in the past. This study is based on β-amyloid hypothesis, with Aβ as the breakthrough point, at the same time including music therapy as an intervention to the treatment of AD. We will delve into different electro-acupuncture treatment guided by Dredging Governor Meridian and Awakening Mind Method for Aβ production means and clear way in frontal cortex of AD model, as well as Compare the curative effect of pulse electro-acupuncture and music electro-acupuncture differences, in order to provide a scientific basis for its clinical application of music electro-acupuncture treatment of AD and other brain-related diseases.2MethodsIn this study, SAMR1and SAMP8mice were used as the research object. They were randomly divided into blank control group (B group), model control group(C group), drug control group(D group), music control group(M group), pulse electro-acupuncture group (PE group) and music electro-acupuncture group(ME group). Morris Water Maze Test was used to detected the learning memory ability of mice, and then compared the influence of two kinds of electro-acupuncture therapy for the ability of learning and memory; Nissl staining was used to observe the pathological changes of mice frontal cortex neurons, and then compared the effect of different therapy; Immunohistochemistry and western blot were used to assay the expression levels of Aβ40and BACE1in frontal cortex, as well as Real-time PCR was used to measure the expression of BACElmRNA, so as to compare the effect of different therapy for Aβ generation; Immunohistochemistry and western blot were used to assay the expression levels of IDE, NEP, LRP1and RAGE in frontal cortex, so as to compare the effect of different therapy for Aβ clearance.3Results3.1Compared with B group, escape latency period baseline was higher and it was not significantly reduced after five days training, as well as total distance of swimming baseline was lower and continued to drop in C group. Escape latency period in groups of D, ME and PE shortened with the increase of training time, especially ME group, but it did not change significantly in M group. Total distance of swimming in ME group was significantly longer than in C group, also longer than D and PE group in some points. In the experiment of space exploration, Search strategy in C group was mostly random and with no obvious bias, as well as the original platform quadrant residence time and swimming distance dropped significantly compared with B group; D, ME and PE groups were better than C group, and ME group was the same as D group but a litter better than PE group.3.2Nissl staining showed frontal cortex pyramidal cells in B group arranged closely and orderly. But in C group, the pyramidal cells in frontal cortex was disorganized or wrinkled like, even incomplete form. Moreover, the number decreased significantly. In addition to the M group, the situation in groups of D, ME and PE were better than in C group.3.3Compared with B group, Aβ40expression level of frontal cortex in C group was significantly increased. Compared with C group, it was decreased obviously in groups of D, ME and PE except M group. Compared with D group, ME group was no significant difference, but PE and M groups were still significantly higher than it. There was no significant difference in Aβ40levels between ME group and PE group.3.4Compared with B group, BACE1mRNA relative expression and BACE1expression levels of frontal cortex in C group were significantly increased. Compared with C group, both in groups of D, ME and PE had different degrees of reduction and the difference was significant. ME group was the same as D group but a litter better than PE group.3.5Compared with B group, expression level of IDE and NEP in frontal cortex in C group was significantly decreased. Compared with C group, both in groups of D, ME and PE had a certain degree of improvement. ME group was better than D and PE groups in terms of regulating the expression of IDE, and there was no significant difference in regulating the expression of NEP between ME group and PE group, but both were not as good as D group. Both IDE and NEP in M group were not statistically significant.3.6Compared with B group, expression level of LRP1in frontal cortex in C group was significantly decreased, but RAGE significantly increased. Compared with C group, both in ME group and PE group were significantly improved, and ME group better than PE group. However, both LRP1and RAGE in groups of D and M were No obvious change.4Conclusions4.1Both pulse electro-acupuncture and music electro-acupuncture treatment guided by Dredging Governor Meridian and Awakening Mind Method can improve the learning and memory disorders of S AMP8mice with AD feature.4.2Both pulse electro-acupuncture and music electro-acupuncture treatment guided by Dredging Governor Meridian and Awakening Mind Method can repair the damage to the frontal cortex neurons of SAMP8mice with AD feature, thus play a role in protecting neurons. In addition, they can reduce the expression levels of Aβ40and correct Aβ metabolic disorder. So they have a role in the treatment of AD.4.3Electro-acupuncture treatment guided by Dredging Governor Meridian and Awakening Mind Method play a role in correcting A(3metabolic disorders through two ways. On the one hand, it can restrain the generation of A(3by reducing the expression levels of BACE1;on the other hand, it can promote the clearance of Aβ by increasing the expression levels of IDE and NEP which belong to Aβ degrading enzyme and regulate the content of LRP1and RAGE which belong to A(3protein related transporters.4.4By means of repairing the damage to neurons and correcting Aβ metabolic disorders in the frontal cortex, both pulse electro-acupuncture and music electro-acupuncture treatment guided by Dredging Governor Meridian and Awakening Mind Method can improve the learning and memory disorders of SAMP8mice with AD feature. There is no difference between the two treatments in adjusting individual indicators, but music electro-acupuncture treatment has more advantage on the whole. |
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