Preparation Of Polyelectrolyte Multilayer Janus Particles For Photo-thermal Therapy

Polyelectrolyte multilayer(PEM)composite microparticles are widely used in the biomedical field as drug carrier due to the small size of microparticles,high possibility of physical and chemical modification on top of the multilayer membrane.Camouflaging the polyelectrolyte multilayer using biological membranes can improve the biocompatibility and reduce risk of being recognized and consumed by macrophages.It also allows for taking advantage of specific functions of membrane proteins to realize specific recognition patterns and aid aggregation on diseased tissue,realize the targeting therapy.Photosensitizers,such as micro-sized gold shell and gold nanorods,have photothermal effect in the area of near-infrared light.The introduction of photosensitizer into the PEM helps photothermal therapy based on PEM composite microparticles.However,the structure and function of drug carriers applying to thermal treatment at present research stages showed poor control ability,it aims to builtup complex structure and functional carriers in the future.This paper aimed to buildup Janus polyelectrolyte multilayer composite particles coated by cell membrane which is modified by photosensitizer and magnetic.The fabrication is on the basis of layer by layer self-assembly method.The PEM is first assembled via electrostatic as well as hydrophobic forces between silica substrate and eco-friendly polyelectrolytes.Then THP-1 cells are cultivated in vitro by simple method of membrane assembly on the polyelectrolyte multilayer.THP-1 cells show a significant recognition to Hela,HepG2,A-549 cancer cells in co-culturing tests.By modifying THP-1 cell membrane asymmetrically onto the PEM,the bionic Janus capsules are allowed specificity recognition capabilities and verify the directivity of phagocytosis by the cancer cell.Near-infrared light sensitive designs are achieved by gold shell forming on the PEM by sputtering,or adding gold nanorods into the PEM film.The clinical laser power(23.6 mW/μm2)is used to kill the cancer cells by exposing the bionic Janus capsule-cancer system.The gold nanoparticles utilized for harvesting 800 nm near-infrared light are produced by a seed growth method.The gold nanorods are modified into the PEM wall of capsule to increase its quantity in the area of photothermal therapy.Combining the magnetic Fe3O4 nanoparticles with photosensitizer using layer by layer method,the prepared PEM composite particles show outstanding achievement in animal photothermal therapy.Magnetic fields can be used to guide the movement of the composite particles,which can be ordered and enriched in the photothermal treat area to realize a body friendly and efficient heat treatment.Photothermal therapy based on PEM composite particles are also significant important for animal wound sealing.Unlike the currently employed color agents and metal nanoparticle dyes,the depth of the wound and the blood flowing out of the wound have no limitation on the application of the composite particles.Photothermal tissue welding can restore a large mechanical force due to magnet controlling.Animal tests as well as histological essays proved that the heat treatment supported by the composite particles can effectively cure the wound,better than that of nanoparticles glue or surgical suture.In conclusion,this article makes use of layer-by-layer assemble method and biomimetic design to control the properties of PEM complex particles,prepared a series of polymer complex particles that possess biocompatibility,special recognition,drug controlled release and photothermal therapy.We study the application of PEM complex particles in photothermal therapy in cancer and mouse tissue wound.New complex materials system based cell membrane and PEM films have promising application in biomedical researches.