Establishment Of A Database For Permanent Teeth Collected In Chinese Populations

Part1Establishment of a database for permanent teeth collected in Chinese populationsObjectives:To establish the Chinese digital permanent teeth database in order to research root canal morphology and fulfill data management, query and other functions.Methods: Chinese permanent teeth were collected, each tooth had a serial number. Micro-CT was used to scan all the teeth, and the scanning resolution was30-37microns.VGStudio MAX and3D-Doctor were used to do image segmentation,3D reconstruction of the external and internal of teeth structure.The cross section,root canal system and tooth contour animations were made.Microsoft Visual Studio2005was used as system development platform, C#was the system development language.In the browser/Server mode (B/S mode), SQL Server2008database management software was used to establish a database for permanent teeth collected in Chinese populations.Results:3309permanent teeth were collected, each tooth had a serial number according to its position. After Micro-CT scanning, the teeth samples were stored in a sample cabinet. With the use of VGStudio MAX and3D-Doctor,3D reconstructions of tooth contour and root canal system had been completed. Cross section animations were made from the crown to the apex.3D reconstructions of tooth contour and root canal system were rotated clockwise to make movies.Each tooth had3movies to be observed from outside to inside, from top to bottom.The Chinese permanent teeth visual database management system was established successfully. System mamagement fuctions including parameter settings, permission settings and user information management were achived.Dental records management functions including tooth records, file information, record retrieval and file retrieval were also achieved.ConclusionsrThe information of Chinese digital permanent teeth database was rich.The database could manage data effectively and assist in dental anatomy teaching and clinical root canal treatment. Part2The interactions of Mg2+and Zn2+containing silicate bioceramics with stem cells and bacteriaObjectives:The objective of this study was to compare the interactions of Mg2+and Zn2+containing silicate and phosphate bioceramics with stem cells and bacteria.Methods:SEM and EDS were used to detect the surface morphology and composition of β-TCR,DIOP and HT.ICP-AES was used to measure ion concentrations in extract solutions.PDLCs and BMSCs proliferaion was determined by MTT assay.The relative expression of ALP and OCN genes were detected by real time-qPCR.Antibacteria tests against planktonic Enterococcus faecalis and biofilm were done by CFU counting and CLSM.Results:DIOP and HT were superior to β-TCP in either enhancing PDLCs proliferation or the antibacterial effect. The three materials had similar stimulation on the growth of BMSCs. DIOP and HT were comparable to β-TCP on osteogenic/cementogenic differentiation of PDLCs. Silicate-based materials had better osteostimulation of BMSCs than phosphate-based material.HT antibacterial efficacy against E.faecalis was slightly inferior to Ca(OH)2.Conclusions:Mg2+and Zn2+containing silicate materials have great potential use for healing and tissue regeneration in apical periodontitis.