Study On Rapid Detection Technology Of Blood Biochemical Parameters

Blood biochemical test is a common medical diagnosis.Rapid detection palys an important role in hospital emergency,outdoor first aid and other occasions to get the patient’s first-hand information.The development of a blood biochemical rapid detection instrument has important significance.This article mainly studies the rapid detection technology for blood biochemical parameters.Detection objects including blood electrolytes,blood gas and blood glucose.The research of this paper mainly includes two parts.First,the detection of the object for a single parameter detection.In this paper,low cost,mass production of screen printing carbon paste electrode is used as the base electrode.Solid state conductive polymer is used as the internal charge electrolyte to make all-solid-state ion-selective electrode and all-solid external reference electrode.It’s easy to transport and storage and prepared for the follow-up multi-parameter detection.The electrochemical instruments were used to test the single detection object,including sodium ion,potassium ion,calcium ion,chloride ion,pH and carbonate ion.The calibration curve,repeatability and consistency for each parameter were recorded.The relative standard error of the measurement results is 0.2%-6.5%.Nernst slope is close to the theoretical value and the consistency is perform well for the standard deviation is between ±1.5-2.9 mV/decade.Prepared of glucose oxidase-based enzyme electrode to detect the blood glucose.SD = 0.22 mmol/L<0.42 mmol/L when the concentration was less than 5.5 mmol/L.The CV was 1.4%-2.2%<7.5%when the concentration was>5.5 mmol/L.The results meet the blood glucose meter standards.In addition,a non-destructive detection method of human blood glucose based on near infrared spectroscopy was proposed.Subjects were given glucose.The subjects’ palm were scanned 7 times near in 1.5 hours with infrared spectroscopy.At the same time,the blood glucose meter was used to test the precise blood glucose concentration.A total of 42 samples were obtained from 6 subjects.Modeling using partial least squares.The experimental results show that the precision of individual modeling prediction(0.40 mmol/L)is better than the overall modeling prediction(0.79 mmol/L).This method can be used for non-destructive testing of blood glucose.Second,the seven test electrodes in the first part were integrated into the same chip.A multi-parameter blood biochemical sensor was prepared.The sensor was combined with self-made portable electrochemical analyzer for the rapid parallel detection of blood biochemical parameters.The test of multi-parameter biochemical sensor is divided into two stages:1.The mixed solution of different concentration detection objects is measured and analyzed.The overall test time is less than 3 minutes,and the correlation of the seven parameters is more than 0.961.2.Measurements were made in real blood samples.The measured accuracy were close to the instrument required values.As the actual blood sample composition is more complex and the interference is large.The results slightly worse than the instrument standard.There is still large improvement space.In this paper,the multi-parameter blood detection sensor has advantages of fast,convenient,low cost,stable performance and so on.This paper focuses on the rapid detection of blood biochemical components,including the design,preparation and measurements of blood ions(sodium ion,potassium ion,calcium ion,chloride ion),blood gas(pH,carbonate ion,carbon dioxide partial pressure)and blood glucose,as well as near-infrared blood glucose testing.The multi-parameter chip integration and its test in mixed solution and blood were also studied.The experimental results show that the proposed method is fast,convenient,accurate and stable.