Based On The Biological Characteristics Of Vascular Factors, The Therapeutic Mechanism Of Immune Thrombocytopenia From Spleen Was Discussed

BackgroundThe spleen controlling blood is one of the main physiological function,it refers that qi of spleen can control the blood flowing in the vessels rather than overflowing the vessels.The patients not only feel fatigue,but also suffer slowly bleeding.From the clinic view,we think that the chronic ITP is the main representative disease of the pattern of the spleen failing control blood.In the view from the traditional Chinese medicine theory,some ITP patients treated with the JPYQSX prescription feel better than before and have less bleeding symptoms no matter what's the number of peripheral platelet.According to this,we claim that it's a huge advantage for treating ITP from the view of the spleen function.There is a complex coagulation system in the body,coagulation and anticoagulation dynamic to maintain the blood flow in the blood vessels.It's due to the quality of platelets,the level of clotting factors and the function of the vasoactive substances to keep the balance.In the past,we thought the ITP as the disorder of the immune system,the decreased number of peripheral platelets and bone marrow megakaryocytes dysmaturity.But a lot of patients suffering from bleeding of skin and mucous membranes and internal organs were thought of lack of platelets and low quality of platelets.But we found that some ITP patients treated with JPYQSX prescription felt better than before and had less bleeding no matter what's the number of the peripheral platelets.This condition can't be explained by the theory of the spleen controlling blood improving the platelets,so maybe there is another way to keep the blood flowing in the vessels.We present a theoretical hypothesis that the function of spleen controlling blood not only locking at the organ spleen,but also the combination of the multiorgan function.It is based on the core of the mitochondrial function and energy conversion and the steady state of the brain intestine axis.An active substance secreted by blood vessels is the target of the transmitting and acting information,and also it's the material basis of the quality of the clotting factors and platelets.We forward to do the basic and clinic research based on the hypothesis,in order to find out the acting target of the treatment by using the theory of the spleen controlling the blood.ObjectWe chose the passive immunity method by using the APS serum to set up the combination model of ITP disease and the pattern of the spleen failing to control the blood,in order to study the role of vascular growth factors and vasoactive substances.We will use the JPYQSX prescription to treat different groups,to find the value of the vascular related factors.MethodWe used the APS serum to setup the combination model of ITP disease and the pattern of the spleen failing to control the blood.We set normal group,model group,prednisone group and JPYQSX group.We did nothing to the normal group except the water and food,we injected the other group mice with diluted 1:4 APS serum.We repeated the injection every day until the end.On the 8th day we fed the mice 0.1ml/ml medicine in the model group,prednisone group and JPYQSX group.On the 16th day,we tested the vaso-active substances in each group by using the ELISA method.We also tested the vascular growth factors by using the HE stained and calculated the expression.The results shown as the x ± s,T test was used for statistical analysis,p<0.05 was statistically significant.ResultThe results of above groups is listed below:?ET-1:Compared with the normal group,the result of the ET-1 in model group,prednisone group and JPYQSX group is lower(p<0.01).The content of endothelin-1 in the prednisone group was significantly lower than that in the model group(p<0.01),but there was no significant difference in the JPYQSX group and model group(p>0.05).The trend of decreasing content was consistent with that of prednisone group.?vWF:Compared with the normal group,the content of vWF in the model group,prednisone group and JPYQSX group was significantly lower(p<0.01).But there was no difference between the prednisone group and JPYQSX group(p>0.05).?VCAM-1:Compared with the normal group,the content of VCAM-1 in the model group,prednisone group and JPYQSX group was significantly lower(p<0.01).Compared with the model group,the content of VCAM-1 in the prednisone group was significantly lower(p<0.05).?TM:Compared with the normal group,the content of TM in the model group,prednisone group and JPYQSX group was significantly lower(p<0.05).There was no significant difference in the prednisone group and model group(p>0.05),but it showed an increasing trend.But compared with the model group,the concentration of TM in the JPYQSX group continue to decrease,and there was significant difference between the two group(p<0.05).?NO:Compared with the normal group,the concentration of NO was significantly lower in the model group,prednisone group and JPYQSX group(p<0.05).The concentration of NO in the prednisone group and JPYQSX group compared with the model group,there was no significant difference(p>0.05).? NOS-3:Compared with the normal group,the concentration of NOS-3 was significantly lower in the model group,prednisone group and JPYQSX group(p<0.05).But there was no difference in the group by treating with prednisone and JPYQSX compared with the model group(p>0.05).?TXA2:There was no difference between normal group and model group(p>0.05),but after treating with the prednisone and JPYQSX,the concentration of TXA2 was higher than normal group and model group(p<0.05).?PGI2:Compared with the normal group,there was no difference in the model group,prednisone group and JPYQSX group(p>0.05).Compared with the model group,there was still no difference after treating with prednisone and JPYQSX(p>0.05).?VEGF:The content of VEGF in the model group and prednisone group was significantly lower than that in the normal group(p<0.05).After treating with JPYQSX,there was no difference between the JPYQSX group and normal group(p>0.05).?bFGF:Compared with the normal group,the content of bfgf in the model group,prednisone group,JPYQSX group was significantly lower than that in the normal group(p<0.05).But there was no difference in the prednisone group and JPYQSX group compared with the model group(p>0.05).Conclusion1.We successfully setup the ITP disease mouse model by using the APS serum,the best concentration is 1:4.And the model meets the standard of the ITP disease and the pattern of the spleen failing to control blood.Until now it is the simple and useful model for research.The JPYQSX prescription can improve the number of the platelets and reduce bleeding symptoms.2.The factors(ET-1,NO,NOS3,TM,vWF,VCAM-1,VEGF and bFGF)involved in the ITP disease.The factors(TM,TXA2,VEGF)play an important role in stopping bleeding after treating with the JPYQSX prescription.It implies that the factors(TM,TXA2,VEGF)are the main substances,because they can explain the theory of the spleen controlling blood which is playing an important role in the ITP disease.