| The excellent electrochemical and optical properties of nanomaterials make them widely used in biochemical analysis,medical test,and environmental monitoring.The electrochemical coupled localized surface plasmon resonance technology based on noble metal nanoarray sensor can effectively realize the joint transmission of electrical signals and optical signals by applying electrochemical excitation on the nano-plasma to modulate the surface electron distribution density.It has brought the advantages of high sensitivity,high reliability and high stability for biosensing detection.In addition,with the increasing demand for portable,miniaturized and intelligent sensing systems in point-of-care testing,smartphone has played an increasingly important role in mobile sensing due to its advanced computing power,high-definition imaging function and open source operating system.In general,smartphone was used as detection platform to sense electrochemical signals and optical signals.Among them,electrochemiluminescence,as an electro-stimulated optical radiation technology,shows strong anti-interference ability in sensing detection,which is conducive to building an integrated detection platform on smartphone and has broad application prospects in biosensing detection.In this paper,the electrochemical coupled optical sensing platform was constructed based on nanoarray,graphene oxide,nanopores and graphene quantum dots for the biosensing detection of thrombin,sialic acid,glucose,cysteine,trinitrotoluene and Escherichia coli.And the electrochemiluminescence detection system was built on smartphone to realize the sensing applications of fingerprints imaging.The main contents of this paper are as follows:(1)Designed and constructed the nanoarray-based electro-optic coupling sensing system.Based on the electrochemical and localized surface plasmon resonance characteristics of the nanoarray sensor composed of periodic nanoparticles,the detections of thrombin and sialic acid were studied.Nanocup array was fabricated by nanoimprinting technology,and gold nanoparticles were deposited on the array by electron beam evaporation to form gold nanocup array sensors.Then a "sandwich structure" was constructed by self-assembly technology,with polyethylene glycol,thrombin-specific polypeptides and albumin modified on the sensors.Finally,electrophoresis-enhanced localized surface plasmon resonance was used for high sensitivity and high specificity thrombin detection.In addition,silver nanoparticles were deposited on gold nanocone array by electrochemical reduction deposition to form gold/silver nanocone array sensors.Then mercaptophenylboric acid was modified on the sensors by covalent binding.Finally,high stability and high selectivity sialic acid detection was achieved by linear sweep voltammetry combined localized surface plasmon resonance technology.Dynamic electro-optical spectroscopy has been realized on the nanoarray sensor,which opened new horizons for the research of high efficiency and high sensitive biosensing detection.(2)Designed and construncted the graphene oxide-based electrochemical/optical sensing system.Based on the electrochemical and optical properties of graphene oxide nanocomposites,the detections of glucose and cysteine were studied.Graphene oxide/aminophenylboric acid nanocomposites were prepared by amide dehydration condensation reaction.Then,the nanocomposites were modified on the screen printed electrode by one-step electrochemical deposition and reduction.Finally,high sensitivity and high stability glucose detection was achieved by electrochemical impedance spectroscopy.In addition,graphene oxide/gold nanoparticles were prepared by thermal reduction method and used as cysteine optical sensing probes.Then multimode colorimetric analysis systems of RGB,CMYK,HSV and HSL were built on smartphone to realize rapid cysteine detection.The combination of graphene oxide nanocomposites and smartphone-based sensing platform provided the support for the building of convenient and efficient analysis system.(3)Designed and constructed the smartphone-based electrochemiluminescence sensing system.Based on the enhancement of luminescence signals by nanopores and graphene quantum dots and the combination of smartphone-based electrochemiluminescence system,the detections of trinitrotoluene and Escherichia coli were studied.The USB-OTG interface on smartphone was used to provide electric excitation,camera to capture dynamic luminescence,RGB,HSV and gray analysis algorithm to extract luminescence signals.Then,nanopores with ion-selective channel were modified on the screen printed electrode to form the nanopores-enhanced luminescence sensor.Finally,polypeptides were immobilized on the sensor as the sensitive layers to realize the detection of trinitrotoluene with high specificity and high sensitivity.In addition,graphene quantum dots/silver nanoparticles complexes were prepared by thermal reduction method as electrochemiluminescence enhancers.Then,Escherichia coli antibodies were immobilized on the indium tin oxide electrode to form the sensitive detection sensor.Finally,quantitative detection of Escherichia coli was realized on the smartphone-based electrochemiluminescence system.The application of nanomaterials on the smartphone-based sensing platform to assist the electrochemiluminescence detection would be helpful for the advancement of mobile biosensing detection.(4)Designed and constructed the fingerprints imaging and sensing system on smartphone.Based on the sensitivity of electrochemiluminescence to the spatial luminescence distribution,fingerprints imaging analysis and biochemical sensing were carried out on the smartphone-based system.Via the built-in function module,an electrochemiluminescence detection platform integrating electrochemical excitation and optical analysis was realized on smartphone.Then,color analysis,gray and binarization processing of fingerprints electrochemiluminescence images were carried out to obtain fingerprints details information.Finally,nicotine enhanced luminescence and trinitrotoluene quenched luminescence on fingerprints were also detected on smartphone.As fingerprints have been widely used to unlock smartphone,fingerprints imaging and sensing on smartphone will greatly promote the development of personal medical detection and public security defense. |
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