LncRNA NEAT1 Activates The Wnt Signaling Pathway By Binding MiR-98-5p To Promote The Development Of Gastric Cancer

Background:Gastric cancer is a very common in China,and its morbidity and mortality are second of the malignant tumors.Recent studies have shown that nuclear enriched transcription 1(NEAT1)is involved in the development and progression of various tumors,while how it effects on gastric cancer and its mechanism are still unknown.Methods:qRT-PCR,RNA interference,cell proliferation test and cell migration test were used to analyze the expression of LncRNA and NEAT1 in gastric cancer and gastric cancer cell lines and the influence of gastric cancer cell proliferation and migration.In addition,qRT-PCR and double luciferase reporter assay were used to investigate the correlation between NEAT1 and miR-98-5p.Results:The expression of LncRNA NEAT1 was up-regulated in gastric carcinoma tissues and cell lines.Over expression of LncRNANEAT1 enhanced the proliferation of gastric cancer cells and promote their migration,further study found that the expression of LncRNA NEAT1 could directly bind and inhibit miR-98-5p,released the targeted inhibition of EZH2,and activated Wnt signaling pathway,then promoted the expression of downstream genes cyclin D1 and c-myc.Conclusion:LncRNA NEAT1 can increase the expression of EZH2 and activate the Wnt pathway to promote the proliferation and migration of gastric cancer cells by binding to miR-98-5p.This will provide a new theoretical basis for the development of targeted drugs for the treatment of gastric cancer by inhibiting the expression of LncRNA NEAT1.Background This study was conducted principally to predict and to determine the risk stratification for lymph node metastasis(LNM)in early gastric cancer(EGC)with mixed histology.Methods We retrospectively collected the data from 967 patients,who underwent gastrectomy for EGC,and then we analyzed the relationship between the clinicopathological factors and the LNM by univariate and multivariate logistic regression analyses.Results LNM was confirmed in 12.1%(117/967)overall.Young age,tumor size,depth of tumor,ulcerative findings and lymphovascular involvement were found to be associated with LNM for EGC(P<0.05).Multivariate analysis revealed that tumor size(odds ratios(OR)1.94,95%confidence intervals(CI)1.23-3.05),depth of tumor(2.31,95%CI 1.33-4.02),young age(≤40year)(5.42,95%CI 2.06-14.25),ulcerative findings(2.93,95%CI 1.08-7.96),lymphovascular involvement(6.00,95%CI 3.05-11.73)and mixed predominantly differentiated(MD)(2.74,95%CI 1.46-5.13),mixed predominantly undifferentiated(MU)(5.54,95%CI 2.78-11.06),pure undifferentiated(PU)(3.39,95%CI 1.68-8.82)were independent risk factors for LNM(P<0.05).Conclusions We recommend that endoscopic dissection(ER)be safely applied for EGC when tumor is confined to muscularis mucosa(pTla/M),pure differentiated(PD)and without lymphovascular involvement and ulcerative findings.Background Alcohol intake has been suggested to have an impact on the development of many chronic diseases.How alcohol intake may modulate risk of Helicobacter pylori(H.pylori)infection,however,remains a subject open for investigation.A dose-response meta-analysis was performed of epidemiological studies to better quantify this relationship.Materials and methods Twelve observational articles were identified.The summary odds ratio(OR)and confidence intervals(CI)were calculated for alcohol drinkers vs non-drinkers.The summary OR estimates were obtained using the random-effects model and dose-response meta-analysis.Sub-group and sensitivity analysis were also conducted.Results The summary OR was 0.78(95%CI=0.69-0.89).The dose-response analysis demonstrated that for drinkers of 10,15,30,60 and 96 g/day alcohol intake,the estimated ORs were 0.80(95%CI=0.76-0.85),0.79(95%CI=0.75-0.84),0.83(95%CI=0.78-0.87),0.85(95%CI=0.78-0.93)and 0.87(95%CI=0.70-1.06),respectively,compared to non-drinkers.The inverse relationship between alcohol intake and H.pylori infection was consistent,regardless of sex,age,geographic areas,detection methods or beverage types.Conclusion Evidence from these observational studies suggests that moderate alcohol intake is associated with a reduction in H.pylori infection of 22%and may facilitate elimination of H.pylori.