Study On The Mechanism Of The Modified Simiao Pills Based On The Relationship Between Uric Acid And Blood Lipid Metabolism

Objective1.Through the retrospective analysis,observe the correlation betweenhigh uric acid hematic disease and fat metabolism disorders among the people in the check-up crowd,based on the relationship between uric acid and lipid metabolism,explore ways to provides the basis for treatment of high uric acid hematic disease traditional Chinese medicine and mechanism.2.Through the animal experiment research,observation been used traditional Chinese medicine compound modified Simiao pills for high uric acid hematic disease model rats blood uric acid,blood lipid level,uric acid transport and discharge,high uric acid and lipid metabolism pathway and regulation mechanism,the influence of clinical commonly used in lowering uric acid and drug benzene bromide Malone contrast both in uric acid and lipid metabolism,so as to jointly from uric acid and lipid metabolism regulation approaches and subtract modified Simiao pills on the mechanism of action of high blood uric acid and lipid metabolism.Methods1.Check-up crowd correlation between high blood uric acid and lipid metabolism disorders were retrospectively analyzed,with the aid of guangdong hospital of a medical records management system and clinical inspection system,and draw on June 1,2017 to January 31,2018 in guangdong hospital check-up crowd ersha island branch medical center archives and blood lipid,uric acid,such as biochemical test results,set up the database,by using the statistical software,IBM SPSS Statistics 22 for the crowd high uric acid hematic disease,hyperlipidemia prevalence,and the correlation of high blood uric acid and lipid metabolism disorder were analyzed.2.Based on the relationship between uric acid and lipid metabolism of flavored modified Simiao pills shot blasting mechanism research: the SPF Wista 46,only in the male rat body weight to 200 + 20 g;In SPF animal room 25 ℃,relative humidity 50%~ 60%,adaptive feeding 1 week,10 were randomly divided into blank group,giving saline lavage;Module,36,giving oxygen oxazine acid potassium 750 mg/kg daily a lavage,respectively,prior to the building mode,orbital venous blood every 7 days after the detection of blood uric acid levels,to build model rats blood uric acid level is 20% higher than that of blank group,determine ChengMo,and 10 were randomly divided into model group,modified Simiao pills group and 14,benzene bromide Malone group of 10,Interventions: after the success of the model,each group were given different interventions,1 times a day.The blank group: normal saline lavage,;Model group: oxygen oxazine acid potassium 750 mg/kg lavage,benzene bromide Malone groups: oxygen oxazine acid potassium 750 mg/kg + 20 mg/kg lavage,add and subtractmodified Simiao pills bolus group: oxazine acid potassium 750 mg/kg + modified Simiao pills group 20 g/kg to fill the stomach,a total of 14 days.3.By using Excel software to record the data of experiment,by using the IBM SPSS Statistics and statistical software for statistical analysis,measurement data with mean + /-standard deviation,according to comparison between multiple sets of single factor analysis of variance,the variance with Welch revised when not neat,with a range of variance under assumptions Dunnett ’s T3 compared between groups,P < 0.05 for statistically significant.Count data by linear regression analysis between uric acid and blood lipid,gender,age,etc,the relationship between trend is same as related to the trend of different negative correlation,the regression coefficient by t test,comparison form than the comparison group with inspection.Results1.The medical people high blood uric acid and lipid metabolic disorder correlation were retrospectively analyzed: participants included 3000 cases of physical examination,high uric acid hematic disease diagnosis standard for 850,28.3% of this group of check-up crowd;Hyperlipidemia diagnosis standard for 964,32.1% of this group of check-up crowd,high uric acid hematic disease accompanied by at least one lipid was abnormal,710 people,23.7% of this group of check-up crowd.83.52% of this group of high uric acid hematic disease crowd.Of 710 patients with high uric acid hematic disease accompanied by at least one lipid abnormal data for further analysis,according to according to the 2017 "China high uric acid hematic disease related disease diagnosis and multidisciplinary experts consensus standards,based on blood uric acid in 420 umol/L,hierarchical analysis of this group of patients,Found high uric acid combined hyperlipidemia in the crowd,the youngest 16 years old,biggest 88 years old,the average age was 46.83 + /-13.47 years old,male 532,female 178 people,the male sex ratio at 74.92%,women accounted for 25.08%;TC was abnormal(27.7%),LDL was abnormal(59.7%),HDL was abnormal(8.6%),TG(77.5%),abnormal blood uric acid in 420 umol/L account for 86.9% or more.TC and LDL were abnormal,118 people,accounting for 16.62%.UA 420 umol/L OR more closely associated with abnormal LDL,UA 420 umol/L OR combined probability of high LDL is UA < 420 umol/L of 2.5 times(OR = 2.547;95% CI = 1.630 3.979).Uric acid is closely related to the level of TG and uric acid in 420 umol/L OR higher,the probability of merger high TG of UA < 420 umol/L is 5.4 times(OR = 5.359;95% CI = 3.394 8.463).Uric acid is closely related to HDL levels,420 merger of uric acid < 420 umol/L HDL anomalies(< 1.04);420 merger of uric acid 420 umol/L or higher HDL exception.2.The animal experiment resultsHigh uric acid: to oxygen oxazine 14 days old rats blood uric acid potassiumlavage appeared rising trend,21 days to build model rats blood uric acidincreased significantly in the blank group(P < 0.05),plus or minus four ratkidney OAT1 express wonderful pill group was obviously higher than that ofblank group,model group,benzene bromide Malone control group,withstatistical significance(P < 0.05);Benzene bromide Malone URAT1 expressionis higher than the blank group,model group,add and modified Simiao pillsgroup,but no statistical significance(P > 0.05).Blood lipid,abnormal blood lipid metabolism in rats,as close to triglycerides and fluctuations in 126.7 + /-18.53 mu molL role;To the blank group,model group,benzene bromide Malone and add and modified Simiao pills oil red staining in the rat liver,prompt high uric acid hematic disease rat liver fatty deposits increased;Acetyl-coa carboxylase,q-PCR detection no statistical difference,WB,according to the results of model group ACC1 expression above plus or minus four miao pills group and benzene bromine Malone,benzene bromide Malone andmodified Simiao pills ACC1 similar expression;ACC1 express the highest ELISA,according to the results of model group,model group and blank group,the add and subtract Simiao pills group and benzene bromide Malone ACC1 express promotion statistically significant(P < 0.05).Q-PCR in detecting acetyl-coa upstream regulation point liver SREBP-1,no difference between groups,(P < 0.05).Through drop uric acid after treatment,blood lipids in rats changes is not statistically significant,but prompt when uric acid decreased cholesterol also reduced.ConclusionU1.High uric acid hematic disease,hyperlipidemia in physical examination population proportion is higher,high uric acid hematic disease associated with dyslipidemia is correlation,especially TG and LDL,prompt high blood uric acid and lipid metabolic abnormalities have a certain relationship,treatment of high uric acid combined hyperlipidemia provides a new direction,among them,the endoplasmic reticulum stress caused by high uric acid might by dash to activate the endoplasmic reticulum stress directly or indirectly,result in disorder of lipid metabolism,and thus become one of the independent risk factors of hyperlipidemia.Four wonderful pill flavored with benzyl bromide Malone function no statistical difference in lowering uric acid,can promote the expression of OTA1,increase the body for uric acid excretion,can inhibit the expression of URAT1,to some extent makes the heavy absorption uric acid to reduce,thus increase the excretion of uric acid.At the same time,add and subtractmodified Simiao pills shot at lower blood uric acid,but also has lower blood lipid.3.Benzene bromide uric acid reduction Malone,although in uric acid indicators,but in terms of the point URAT1,benzene bromide Malone URAT1 is higher,the mechanism remains unclear.4.High uric acid can be used as independent risk factors for lipid metabolic disturbance,its relationship with blood lipid mainly involves the triglycerides,which acetyl-coa carboxylase can be thought of as one of the key points,endoplasmic reticulum stress may be A new research breakthroughs.5.The doctor of traditional Chinese medicine for the treatment of diseases,treatment based on syndrome differentiation and the overall concept,different word with the same disease cure is the application of traditional Chinese medicine dialectical thinking one method of dealing with diseases,high uric acid hematic disease and hyperlipidemia in clinical part can be attributed to the category of traditional Chinese medicine "wet",wet as high uric acid hematic disease,hyperlipidemia,and even one of metabolic diseases treatment breakthrough point of traditional Chinese medicine.6.Due to the limitation of time,this topic in high uric acid by endoplasmic reticulum stress in liver lipid metabolic abnormalities of a part of the research has failed to fully incorporated into to study in the paper,with the ongoing research results improve,there will be a complete essay form new perspectives reflect high uric acid and lipid metabolism relations;Belongs to the animal experiment is given priority to,this study has not been able to incorporate elements such as TCM symptom of study,to be in the future we will further improve the research scheme,add more realistic basis for the development of TCM.