Study On The Effect And Mechanism Of An Xiaoxian’s Academic Thinking On Treating Children Tic Disorder From Liver Wind And Phlegm

Tic disorders are chronic neuropsychiatric disorders are common in children and adolescents,mainly for involuntary,fast and repeated non rhythmic movement and(or)no obvious purpose,sudden onset of vocal tics,the male is more than female,the incidence is increasing year by year,up to 1%～4%,has brought the adverse effect is serious for children learning,health and family life.Professor AN has been engaged in clinical pediatrics of traditional Chinese medicine and Western medicine,scientific research and teaching work for 50 years,with TCM profound theoretical knowledge and rich clinical experience,especially for the treatment of children with tic disorder and obvious effect in pediatric medicine industry enjoys a high reputation.In this study,YGTSS total score,total effective rate and integral progression rate were used to conduct a self controlled study.And an empirical collection and data mining system of TCM was developed by the Chinese Academy of Sciences and the Xiyuan Hospital of the Chinese Academy of science of traditional Chinese medicine(Chinese Academy of Chinese Medicine).The core prescription of dynamic disorder and the method of network pharmacology study the effective component,target and action mechanism of the prescription for the treatment of aspiration disorder,and provide the theoretical basis for the application of the core prescription。1.Professor AN’s experience and curative effect evaluation on treating children tic disorder from liver wind and.phlegm.[Objective]Summary of academic thought and clinical experience of Professor AN in the treatment of children with tic disorders,explain the effectiveness and mechanism of Professor ANin two aspects of macroscopical and microcosmic.[Methods]In this paper,the Yale comprehensive aspiration scale(YGTSS)was used to evaluate the efficacy and safety of the subjects(extracted from September 2012 to September 2015 100 cases of tic disorder diagnosed by Professor ANN 400 diagnostic data),which were treated by Professor An,Professor AN,for a period of 8 weeks.At baseline,second weeks,fourth weeks,and eighth weekends,the results were evaluated.YGTSS total score,total effective rate and integral progression rate were used to conduct a self controlled study to prove its validity from objective aspect.[Results]Chinese medicine treatment of 100 cases in the observation group,39 cases were cured,21 cases were markedly effective,32 cases were effective,8 cases were invalid,the total effective rate was 92%.We can see that both the total score of YGTSS and the rate of progress of integration show that it is effective to treat the disorder of aspiration from the liver wind clip phlegm,and to consult the literature and data of Western medicine and Western medicine for the treatment of twitches,we also conclude that the effect of an effective teacher is better than the treatment of Western medicine.2.The core prescription was extracted by data mining,and the core prescription was analyzed by network pharmacology.[Objective]Using data mining to extract the core prescription,and through the network pharmacology method,the effective component,target target and its mechanism of the core prescription therapy are analyzed.[Methods]In this study,the traditional Chinese medicine experience collection arid data mining system developed by the Chinese Academy of Sciences and the Xiyuan Hospital of the Chinese Academy of science of traditional Chinese medicine(Chinese Academy of Chinese Medicine)was developed.A structured electronic medical record collection template was formulated according to the clinical data collection table.The diagnosis,syndrome,and treatment methods were determined by two classification methods(there were or no),and the symptoms were determined according to the actual conditions.The two classifications(with or without)or multiple classifications(for example,"tongue red","pale tongue,pale tongue,tongue red,tongue red")are used separately.The case information is input to the case collection system to prepare for the next step of data mining.The data entry was carried out by two people and checked two times.Network pharmacology analysis of core prescription drugs:1)construction of the molecular target network of the disorder.By using Drugbank(http://www.drugbank.ca/),Therapeutic Target Database(TTD,http://bidd.nus.edu.sg/group/cjttd/),Comparative Toxicogenomics Database(CTD,http://ctdbase.org/)and other databases,the key words are entered to find the related target of the twitch disorder,and then through protein protein.The protein-protein interaction(PPI),such as HAPPI,IntAct,MINT and DIP,obtained the target pairs of the target interaction target-interaction target,and constructed the protein interaction network of the target target by String 10 software so as to tap the barrier molecular target network.2)Core formulation and target prediction of the core of aspiration disorder.Through the BATMAN-TCM online software(http://bionet.ncpsb.ORG/batman-tcm/),the core prescription drugs are recorded,the effective components are collected,and the potential target targets are predicted.And based on Fang Yi,that is,Tianma,hocho,cicada,chrysanthemum,and chrysanthemum are used to relieve the spasmodic.The pinellia,tuckahoe and calamus can dispel phlegm,the acid jujube kernel,Paeonia lactiflora,and licorice are used to nourish yin and relieve the.spasmodic.The raw dragon bone and the raw oyster are used as a heavy Town,and the core prescription is to expel the spasmodic and dispel the dampness.Phlegm,nourishing Yin,tranquilizing the nerves,relieving spasms,and tranquilizing the nerves for different clusters were analyzed.3)Construction and analysis of network.Use Cytoscape3.5.2 software(http://cytoscape.org/)to carry out network construction,analysis and visualization.We constructed 3 networks:the target network of tic disorders,the ingredient target network of core prescriptions,and the network of target related components,the tic related target network.4)Enrichment analysis,Gene Ontology(GO)enrichment analysis and KEGG metabolic pathway analysis.Based on BATMAN-TCM online software for core formulation of disease enrichment analysis,ClueGO software is used for the GO enrichment analysis and KEGG metabolic pathway analysis of the target drone related target of the core prescription.[Results]1)construction of the molecular target network of the disorder.A total of 35 target targets were found through the database and 535 targets were interacted with the interaction target,which constituted the target target pair of the twitch target to the network.There were 651 target target pairs of the twitch target,and the interaction target could be used to indirectly affect the biologic network of the extraction barrier.At the same time,the most important targets are BDNF,TH,FOS,DRD2,CREB1,NPY,NR3C1,SLC6A4,PRL,DRD4,EGR1,SLC6A3,CASP3,AVP,DRD3,PENK,MAOB,HTR2A,HTR2C,OPRM1,NTRK2.2)Core formulation and target prediction of the core of aspiration disorder.The BATMAN-TCM software is used to obtain the composition target network of the core prescription of the aspiration disorder,which shows that the core prescription can be used in multiple pathways and various diseases through multiple targets and multiple targets.The results of disease enrichment and KEGG pathway enrichment analysis can be seen,Neuroactive ligand-receptor interaction,GABAergic synapse,Serotonergic synap Se,Dopaminergic synapse and other pathways are closely related to tic disorders.3)Construction and analysis of network.There are 3 genes in the four clusters of the four clusters,namely,CACNA2D1,HTR2A,SLC18A2,driving the head wind and relieving spasspasms and recombining with the heavy town.There are 211 genes that drive the head wind to prevent spasmodic pumping and the nourishing Yin Anshen antispasmodic recoincide gene.There are 274 phlegm recoincide genes.There are 153 genes that drive the head wind and stop the spasspasms,the nourishing Yin Anshen antispasmodic and the dampness and the phlegm recombination gene.It shows that the core prescription of the disorder can play the role of different treatments through different targets,and the different treatments can also play a synergistic effect through the same target,which is also very consistent with the traditional Chinese medicine.The principle of compatibility.4)Enrichment analysis,Gene Ontology(GO)enrichment analysis and KEGG metabolic pathway analysis.The drugs in the core prescriptions are butted with the target of the disease of the disease,to explore the efficacy components and targets of each drug.Gastrodia elata can act on the target of 27 diseases through 6 components,and the hooked rungus can act on 17 targets by 18 components.The chrysanthemum can be used in 25 points for 25 targets and cicada to pass through.2 components can act on the target of 24 diseases and the Pinellia ternata can act on 41 target targets by 17 components,Poria cocos can act on 15 target targets by 7 components,and calamus can act on 16 target targets by 9 components,and Paeonia lactiflora can act on 15 target targets through 11 components and licorice can act on 2 by 41 components.1 targets of disease and Zizyphus jujube can act on 97 target targets by 12 components,and oysters can act on 1 target targets by 1 components.The keel does not find the components that can be used for the target of the disease,which may be related to the limitations of the present database.[Conclusion]Through the analysis and analysis of their own front and back control and network pharmacology,it is concluded that an effective,effective,integrated analysis of pharmacophore,the prediction of drug target,and the enrichment of the pathway were preliminarily discussed.The mechanism and access of obstacles,the core prescription mainly through regulating the targets and channels of a variety of neurotransmitters to play the role of treatment,and compared with western medicine,the advantages of traditional Chinese medicine treatment of the disease is:Traditional Chinese medicine treatment can be combined with disease,flexible medication,according to the symptoms of each child,individualized treatment;The common disease common disease attention deficit hyperactivity disorder,anxiety disorder,etc.also have a more obvious effect of treatment or relief,and it has a better effect on the whole body condition of the children.Chinese medicine has obvious clinical effect,little side effects and easy acceptance by children and family members.It lays a good foundation for further study of the disease.