Symbiotic Bateria Of Pregnancy And Infants In Perinatal Period And Their Dependency

Chapter1 Components and variation of microbiota of pregnancy and their infantsObjectives:High-throughput sequencing was used to analyze the components and variation of microbiota of different period in pregnancy and their infants.Method:1.Pregnancy with regular check in 1st affiliated hospital of Jinan University were chosen in our study after informed consents were obtained.Their infants were followed-up to 14 days after birth.2.Feces samples,leucorrhoea samples of pregnancy were obtained in 32-34 weeks and full-term respectively.Meconium samples were obtained.Feces samples of infants were obtained in 3days and 14 days after birth.High-throughput sequencing was used to analyze the components and variation of these samples.Result:1.In phylum,gut microbiota in feces samples of pregnancy are mainly Firmicutes,Bacteroides and Actinobacteria.Vaginal microbiota in leucorrhoea samples of pregnancy are mainly Firmicutes,Actinobacteria and Bacteroides.Gut microbiota in meconium are mainly Preteobacteria,Firmicutes and Actinobacteria.Gut microbiota in feces samples in 3 day and 14 day of infants are mainly Firmicutes,Preteobacteria and Actinobacteria.2.In phylum,Acidobacteria and Chloroflexi from feces of pregnancy in full-term were less than those in 32-34 weeks.In genus,Clostridium_sensu_stricto₁ from feces of pregnancy in full-term were less than those in 32-34 weeks.Parabacteroides from feces of pregnancy in full-term were more than those in 32-34 weeks.In phylum,Firmicutes from leucorrhoea of pregnancy in full-term were less than those in 32-34 weeks.In genus,Lactobacillus from leucorrhoea of pregnancy in full-term were less than those in 32-34 weeks.Acinetobacter and Sphingomonas from leucorrhoea of pregnancy in full-term were more than those in 32-34 weeks.The unique species increased in full-term gut microbiota but decreased in full-term vaginal microbiota.3.In phylum,Firmicutes and Actinobacteria in gut microbiota of infants 3days feces are more than those in meconium.Proteobacteria,Cyanobacteria,Tenericutes,Acidobacteria and Chloroflexi of infants 3days feces are less than those in meconium.Gut microbiota of infants in 3days and 14 days have no significant difference.In genus,Streptococcus,Enterococcus and Bifidobacterium of infants 3days feces are more than those in meconium.Ralstonia,Acinetobacter,Roseburia,Faecalibacterium,Dialister,Blautia,Sphingomonas,[Eubacterium]_coprostanoligenes_group and Ruminococcaceae of infants 3days feces are less than those in meconium.Enterococcus of infants 3days feces are less than those in meconium.Clostridium_sensu_stricto₁ of infants 3days feces are more than those in meconium.The unique species decreased sharply in 3days and 14 days feces compared with meconium.Conclusion:1.Gut microbiota and vaginal microbiota in 32-34 weeks and full-term have significant difference in phylum and genus.The unique species increased in full-term gut microbiota but decreased in full-term vaginal microbiota.2.In phylum and genus,gut microbiota from meconium and feces in 3days have significant difference.In genus,gut microbiota from feces in 3days and 14 days have significant difference.The unique species decreased sharply in 3days and 14 days feces compared with meconium.Chapter2 Components and origin of Placenta microbiotaObjective:High-throughput sequencing was used to analyze the components and origin of placenta microbiota.Method:Placenta samples were obtained and analyzed via High-throughput sequencing.Multiple linear regression were used to analyze the origin of placenta top10 phylum and top30 genus microbiota.Results:1.Placenta is not sterility.Components of placenta microbiota are complicated.The number of unique species in placenta are 5840.2.In phylum,Proteobacteria from placenta were associated with those in vaginal in 32-34 weeks.Tenericutes from placenta were associated with those in gut in 32-34 weeks,as well as in full-term.3.In genus,Clostridium_sensus_stricto₁ from placenta were associated with those in gut in 32-34 weeks.Fusobacterium from placenta were associated with those in vaginal in 32-34 weeks.Sphingomonas from placenta were associated with those in vaginal in 32-34 weeks,as well as gut in full-term.Conclusion:1.Placenta is not sterility.Components of placenta microbiota are complicated and have lots of unique species.2.Placenta microbiota were associated with gut microbiota and vaginal microbiota from pregnancy.And most of association were in 32-34 weeks.Chapter3 Origin of gut microbiota from infants by vaginal deliveryObjective:Dependency of gut microbiota from infants by vaginal delivery with microbiota,vaginal microbiota and placenta microbiota from pregnancy were analyzed to explore the origin of gut microbiota from infants.Method:Multiple linear regression were used to analyze the origin of top10 phylum and top30 genus gut microbiota from meconium,feces in 3days and 14 days.Results:1.In phylum,Actinobacteria from meconium of infants were associated with those in pregnant vaginal microbiota in 32-34 weeks.Acidobacteria from feces of infants in meconium were associated with those in pregnant gut microbiota in full-term.Firmicutes from feces of infants in 3days were associated with those in pregnant gut microbiota in full-term.Tenericutes from feces of infants in 14 days were associated with those in pregnant placenta microbiota.2.In Genus,Enterococcus,Parabacteroides,Dialister,Blautia and Ruminococcaceae in gut microbiota of infants from meconium were assiociated with those in pregnant gut microbiota in 32-34 weeks.Blautia and Ruminococcaceae in gut microbiota of infants from meconium were assiociated with those in pregnant gut microbiota in full-term.Bifidobacterium,Staphylococcus,Clostridium_sensu_stricto₁,Akkermansia and Ruminococcaceae were assiociated with those in pregnant vaginal microbiota in full-term.Coprostanoligenes were assiociated with those in placenta.3.In Genus,Prevotella,Blautia and Ruminococcaceae in gut microbiota of infants from 3days were assiociated with those in pregnant gut microbiota in 32-34 weeks,while Lactobacillus and Bifidobacterium were assiociated with those in pregnant vaginal microbiota in 32-34 weeks.Megamonas,Ruminococcaceae and Blautia in gut microbiota of infants from 3days were assiociated with those in pregnant gut microbiota in full-term,while faecalibacterium and Ruminococcaceae were assiociated with those in pregnant vaginal microbiota in full-term.Staphylococcus and Atopobium were assiociated with those in placenta.4.In Genus,Escherichia-Shigella in gut microbiota of infants from 14 days were assiociated with those in pregnant gut microbiota in 32-34 weeks.Staphylococcus and Megamonas in gut microbiota of infants from 14 days were assiociated with those in pregnant gut microbiota in full term,while Streptococcus were assiociated with those in pregnant vaginal microbiota in full term.Megamonas were assiociated with those in placenta.Conclusion:1.Gut microbiota of infants in meconium and 3days were mostly associated with gut microbiota in 32-34 weeks,gut and vaginal microbiota in full-term.Placenta microbiotas of their pregnancy have less association.2.The associations among pregnancy and infants decreased constantly as time goes on.Chapter4 Therapeutic effect of probiotics in pregnancyObjective:Components of gut microbiota,vaginal microbiota and placenta microbiota of pregnancy,as well as gut microbiota of their infants were compared between probiotics group and control group to explore the therapeutic effect of probiotics.Method:1.Pregnancy were divided into probiotics group and control group randomly.Pregnancy in probiotics group took 2pics Golden Bifid 2 times per day until delivery.Pregnancy in control group took nothing.2.Components of gut microbiota,vaginal microbiota and placenta microbiota of pregnancy,as well as gut microbiota of their infants were compared.Results:1.In phylum and genus,components of gut and vaginal microbiota between control group and probiotic group in 32-34 weeks has no significant difference.2.After taken probiotics,Firmicutes in gut microbiota were more than those before taken probiotics,while Acidobacteria were less.After taken probiotics,Ralstonia and Blautia in gut microbiota were more than those before taken probiotics,while Parabacteroides were less.After taken probiotics,Fusobacteria in vaginal microbiota were more than those before taken probiotics.Sneathia,Prevotella and Veillonella were more than those before taken probiotics.3.In phylum,components of gut and vaginal microbiota between control group and probiotic group in full-term has no significant difference.In genus,Blautia from gut microbiota in probiotic group were more than those in control group,while Roseburia were less.Components of vaginal microbiota between control group and probiotic group in full-term has no significant difference.4.In phylum,placenta microbiota in probiotic group and control group has no significant difference.In genus,Escherichia-Shigella from placenta in probiotic group were more than those in control group.5.In phylum and genus,gut microbiota from meconium,3days and 14 days feces has no significant difference between probiotic group and control group.Conclusion:1.Probiotics in our study can change components of some functional microbiota from gut and vaginal of pregnancy,but can’t change corresponding probiotcs.2.Probiotics in our study can change components of some functional microbiota from placenta,but can’t change corresponding probiotcs.3.Probiotics in our study can’t change components of gut microbiota from meconium,3days and 14 days gut microbiota of infants.