Phosphoglycerate Kinase 1 Gene Knockdown Suppresses The Proliferation?Migration And Invasion,and Promote Apoptosis Of Glioma Cells

Objective Phosphoglycerate kinase 1(PGK1),a glycolytic enzyme,is overexpressed in several carcinomas and plays an important role in their malignancy.However,expression and function of PGK1 in gliomas are unknown.Methods In the present study,we determined PGK1 protein expression in different World Health Organization(WHO)grade glioma tissues and normal brain tissues by performing immunohistochemical analysis.Small-interfering RNA against PGK1(si-PGK1)was synthesized and was transiently transfected into 2 human glioblastoma cell lines U-87 MG and U-118 MG.PGK1 m RNA and protein expression after transfection were detected by performing quantitative reverse transcription-PCR(q RT-PCR)and western blotting respectively.CCK-8 method was used to detect the proliferation of cells after transfection and flow cytometry was used to detect the cell apoptosis after transfection.Effect of PGK1 knockdown on the migration and invasion of U-87 MG and U-118 MG cells was determined by performing transwell assay.Further,effect of PGK1 knockdown on the protein expression of beta-catenin and chemokine receptor 4(CXCR4)was determined by performing western blotting.Results Immunohistochemical analysis detected high PGK1 protein expression in different WHO grade gliomas.Moreover,upregulated PGK1 protein expression was significantly associated with gliomas having advanced WHO grades.Si-PGK1 transfection successfully silenced PGK1 m RNA and protein expression in U-87 MG and U-118 MG cells,as confirmed by performing q RT-PCR and western blotting,respectively.PGK1 knockdown suppresses the proliferation and promote the apoptosis of glioblastoma cell.PGK1 knockdown also suppressed the migration and invasion of U-87 MG and U-118 MG cells and decreased the protein expression of beta-catenin and CXCR4,which promote the invasion and metastasis of many carcinomas.Conclusions Thus,these results suggested that PGK1 played an oncogenic role in gliomas and could serve as a biomarker for predicting the metastatic progression of gliomas.