| Objectives: 1.To investigate the distribution of Chinese medicine Syndrome Elements Distribution in overweight and simple obesity,and provide evidence for clinical syndrome differentiation.2.To explore the mechanism of intervention of Jianpi Tiaogan yin in obesity mice.Methods: 1.Clinical research: To develop a questionnaire of syndrome elements,collect 91 cases of overweight and 115 cases of simple obesity,collect the information of the Chinese medicine diagnosis related to this study,and use the diagnostic criteria of Zhengsu Bianzheng Xue to extract syndrome elements,establish a certificate database,and perform statistical analysis.2.Experimental study: An obese mice model was established,Jianpi Tiaogan Yin and positive control metformin were administered to mice for 6 weeks.The general condition of the mice and obesity-related indicators(body weight,abdominal white fat mass,etc.),glycolipid metabolism indicators(Fasting Blood Glucose,Triglycerides,Total Cholesterol,etc.),white and brown adipose tissue morphology,and White fat brown indicators and changes in gut microbiota were observed to discuss the efficacy of Jianpi Tiaogan decoction to lose weight.Results: 1.Clinical research:(1)Study on overweight population:(1)In terms of eating habits,the proportions of “Partial eclipse Feiganhouwei” accounted for the most.(2)In terms of daily sports,“minor exercise” accounted for the most,accounting for 69.23%.(3)In the distribution of symptoms and signs,in terms of etiology “careless eating(eating too much food)”is the most important factor,constituting a ratio of 78.02%;in terms of sweating,the "sweat" and "heavy sweat days" accounted for the most;in terms of head discomfort,"burnout fatigue" is the most common;in terms of emotion and sleep,"insomnia" and "upset" are in the top two;in terms of physical signs,"obesity" is 61.54%;in terms of tongue coating,the proportion of "tongue pale" and "tongue greasy" accounted for the largest proportion.(4)The proportion of syndrome elements’ distribution was above 60% with spleen,liver,qi stagnation,qi deficiency,wetness and sputum.At the element level,the proportions of Level I and Level II accounted for the largest proportion.In the cluster analysis of syndromes,if they are clustered into 3 categories,category 1 is: qi deficiency,dampness,spleen,sputum,qi stagnation,food product,and liver;category 2 is kidney,lung,and blood stasis;category 3 is stomach,heat.With the increase of clusters,Type 1 can be gradually refined and differentiated into different clusters.(2)Research on simple obese people:(1)In terms of eating habits,the proportions of “Partial eclipse Feiganhouwei” accounted for the most.(2)In terms of daily sports,“minor exercise” accounted for the most,accounting for 59.13%.(3)In the distribution of symptoms and signs,in terms of etiology “careless eating(eating too much food)”is the most important factor,constituting a ratio of 90%;in terms of sweating,the "sweat" and "heavy sweat days" accounted for the most;in terms of head discomfort,"burnout fatigue" is the most common;in terms of emotion and sleep,"Sigh" and "Shenpi" are in the top two;in terms of physical signs,"obesity" is 100%;in terms of tongue coating,the proportion of "tongue pale " and "tongue greasy" accounted for the largest proportion.;in terms of pulses,the "string pulse","slip pulse" accounted for the first two.(4)The proportion of syndrome elements’ distribution was above 60% with Spleen,liver,qi stagnation,qi deficiency,wetness,sputum.At the element level,there are more Class III certificates.For the cluster analysis of syndromes,if they are clustered into four categories,the first category is: spleen,qi stagnation,and sputum;the second category is qi deficiency and wetness;the third category is liver;the fourth category is food product.2.Experimental study:(1)Compared with the NC group,the white fat in the abdomen of the HFD group was significantly increased(P<0.01),suggesting that obese mice induced by high-fat diet have abdominal obesity.Compared with the HFD group,the white fat in the JPTGY group was significantly reduced(P<0.01),and significantly decreased in the DMBG group(P<0.05).Compared with the DMBG group,the white fat decreased significantly in the JPTGY group(P<0.05).Jianpi Tiaogan Yin and metformin could reduced body weight of obese mice(P<0.05),and Jianpi Tiaogan Yin was better than metformin(P<0.05).Compared with NC group,liver weight of HFD group mice was significantly increased(P<0.05);JPTGY group was less obvious than HFD group(P<0.05).Compared with NC group,TG,TC,and LDL-c in HFD group were all significantly higher(P<0.01),suggesting that high fat diet induced mice with elevated serum lipids.Compared with HFD group,the TC in DMBG group and JPTGY group were significantly lower(P<0.01),suggesting that Jianpi Tiaogan Yin and metformin have a better effect on reducing total cholesterol in mice.Compared with the HFD group,the TG in the JPTGY group decreased significantly after treatment(P<0.01),the DMBG group was significantly lower(P<0.05),suggesting Jianpi Tiaogan Yin and metformin can effectively reduce triglyceride in mice,and Jianpi Tiaogan Yin is better than metformin.Compared with HFD group,the LDL-c was significantly decreased in DMBG group and JPTGY group(P<0.01),suggesting that Jianpi Tiaogan Yin and metformin have a better effect on lowering LDL-c.Compared with HFD group,the white adipose tissue of JPTGY group significantly up-regulated the expression of UCP-1 and PGC-1α at the level of protein and gene(P<0.01),and the efficacy was superior to DMBG group(P<0.01).(2)Study of gut microbiota showed that the species richness and diversity of gut microbiota in the JPTGY group was higher than that in the HFD group.The species richness and diversity of gut microbiota in the HFD group was higher than that in the DMBG group,and they was statistical significance.Metformin increased the dysbacteriosis of the gut microbiota while the richness of verrucomicrobiace at the phylum level was significantly higher than that of the other groups.Jianpi Tiaogan Yin increased the richness of the intestinal flora of the obese mice at the phylum level and reduced the abundance of the bacteroidete;the richness of bacteroidales_S24-7_group,lactobacilite were increased at the level of the family.Conclusions: 1.Clinical research: The syndrome elements of overweight and simple obesity are mainly y in the spleen,liver,qi stagnation,qi deficiency,sputum,and dampness.So,the treatment is based on the principles of strengthening spleen Qi,soothing the liver,and removing phlegm.At the level of syndrome elements,overweight patients account for the majority of class I or class II.Class III is more common in patients with simple obesity.It also shows that the simple obese patients are relatively serious and overweight is the early stage of simple obesity;in the overweight stage,the intervention of traditional Chinese medicine can block its development towards simple obesity.2.Experimental study:(1)The intervention of Jianpi Tiaogan Yin in obese mice can significantly reduce the body weight of mice and improve abdominal obesity and glucose and lipid metabolism in obese mice.(2)The intervention of Jianpi Tiaogan Yin in obese in mice may increase the expression of UCP-1 and PGC-1α in white adipose tissue and promote the browning of white fat,which can reduce the weight of obese mice.(3)The intervention of Jianpi Tiaogan Yin in obese mice may increase the richness of gut microbiota in obese mice and regulate the dysbacteriosis of gut microbiota,which can reduce the weight of obese mice and improve the metabolism of glucose and lipid in obese mice. |
PDF Full Text Request