The Mechanisam Of Mechanical Strech Induced Skin Regeneration

Objective: Tissue expansion is a commonly used procedure in plastic and reconstructive surgery.Short-term mechanical stretch can induce skin regeneration,however,in some cases,longterm mechanical stretch may induce some complications,like skin thicking,necrosis,etc.To understand the protential mechanism is realtively important,it would help to find the therapeutic target for clinical use,and to promote skin regeneration capacity.Such therpapy may also help to reduce the prossiblity of mechanical streach induced skin atrophy in order to prevent tissue expansion relationed complications.Methods: Weighted Gene Co-Expression Network Analysis(WGCNA)was ultilized to explore the hub genes within mechanical stretch induced skin regeneration gene network.In vivo and in vitro experiment were done to verify the function of hub genes and their downsteam pathways.Results: Through WGCNA,Cdh1 and CCN1 were found to be the significantly correlated with mechanical force induced skin regeneration.Through in vivo and in vitro intervention,Cdh1 can induce skin keratinocyte dedifferenciated to basal stem cell and gain stem cell phenotype,while CCN1 can promote EMT process to increase the quantitiy of fibroblast,and stimulate collagen synthesis,so as to alleviate the symptoms of skin atrophy.Conclusion: Both Cdh1 and CCN1 are protentially valuable therapeutic target for mechanical streach induced skin atrophy.Further researches will mainly focus on the downsteam mechanism for these two hub genes’ biological function.