Investagation Of Microenvironment Osteogenesis Ability Based On The Cell Aggragation Techenoligy

Recent years bone tissue enginerring research work mainly foucs on the osteogenesis ability of the stem cells,the biocompatibility and mechanical capacity of the scaffold is widly discussed as well.The mechanism of the maintaining and transport of the growth factor are still not clear.Therefore,scientists trying to apply cell aggregate technology into the bone regeneration field.Since then this technology makes a remarkable outcomes,but still,the basic founctinal role that the cell matrx do is doubtable.The microenvironment change during the osteogenesis progress within the cell aggregation needs to be investigated and improved in the future work.In this study,we choose different time points during the cell aggragation inducement and test the molecular level variation of the cell conjounction signal,which will give us a further guidance of the bone regeneration research.And for part four,we developed a novel 3D porous hyaluronic acid-hydroxyapatite(HA-Hap)scaffold,which mimicked the nature bone tissue architecture,so we investigate the potential of using this novel scaffold for bone tissue engineering both invitro and in vivo.With all the results,we can make the summery like below:1.Mesenchymal cells aggregations changed the shape and cell matrix quutity during the growing time,andalso the cell signal expressing when inducing time increased.The best part of the cell aggregation condition to use is the 7 days inducement cell aggregate.2.After cultured cell aggregation invivo for 8 weeks,we found that the cells still diffication osteoblast way,although we already stoped the induction since we implanted in to the mice bady.And the cell layers themselves tends to be reorgniged after 8 weeks.Some part of the aggregate shows a varscular protein expression,which may needed as a nutrition supply.3.By using r-BMMSC cell aggregation to conbined the Ti-implant in order to get an enhanced bone-healing rate in osporosis rat model,we have the results that showed this technical really plays a ourstanding osterogenesis effort in the bone regeneration field.4.A novel 3D porous hyaluronic acid-hydroxyapatite(HA-Hap)scaffold which structurly mimic the nature bone and its biocampactivity has been developed to gives us a productivepotential gone graft material in the large bone defect restorative.