Basic Study On The Application Of Novel Chitooligosaccharide Nanomaterials In The Treatment Of Posttraumatic Osteoarthritis

Objectives: Post-traumatic osteoarthritis is the leading cause of mobility-related disability.It is a common bone and joint disease in the middle-aged and elderly population.Due to its complex pathogenesis and poor prognosis,there is few reliable guideline for clinical diagnosis and treatment.Strategies,common treatments such as medication,joint debridement and replacement have significant defects.In this study,novel chitosan oligosaccharide nanomaterials were used to test the efficacy of novel chitosan oligosaccharide nanomaterials for post-traumatic osteoarthritis and to explore its cellular and molecular mechanisms.Methods: Chondrocytes from rat cartilage tissue were cultured as the in vitro research object.CCK-8 technique was used to detect the effect of chitosan oligosaccharide and chitosan oligosaccharide@silica sustained-release material on the proliferation of chondrocytes.TUNEL apoptosis assay was used to detect the effects of apoptosis on chondrocytes.In vivo experiments were performed using an animal model of rat ligament surgery,followed by injection of normal saline,chitooligosaccharide or chitosan oligosaccharide@silica sustained-release material.The degree of knee joint recovery was evaluated using a micro-CT system.In addition,we also explored the possibility of establishing a new post-traumatic osteoarthritis model with gold nanorods combined with NIR lasers.Results: Chitosan oligosaccharide could significantly promote the proliferation of chondrocytes and reduce the damage of IL-1β on chondrocytes.However,when its concentration was too high,the therapeutic effect would be reduced.In animal experiments,chitosan oligosaccharide treatment was used directly,the effect was not satisfied,and the dose effect was not obvious.Chitosan oligosaccharide@Si O2 nanomaterials have good biocompatibility and long-term safety guarantee in the body.In vitro,chitosan oligosaccharide@silica sustained-release nanomaterials can significantly promote chondrocyte proliferation and reduce IL-1β damage to chondrocytes.In animal experiments,chitosan oligosaccharide@silica sustained-release nanomaterials significantly improved the prognosis of traumatic arthritis.Gold nanorod injection combined with laser irradiation successfully established a new,accurate and controllable model of osteoarthritis.Conclusions: Our results suggest that chitosan oligosaccharide has a positive effect on proliferation and anti-apoptosis of chondrocytes,but it can not be directly used for clinical treatment of osteoarthritis.Chitosan oligosaccharide@Si O2 nanomaterials are biosafe and both in vivo and in vitro experiments have proven oligosaccharide@Si O2 nanomaterials to be useful in the treatment of post-traumatic osteoarthritis.In addition,the osteoarthritis model established by gold nanorod injection combined with laser irradiation is an accurate,effective and stable modeling method.