Enhancement Of Rotator Cuff Tendon-Bone Healing Using Combined Aligned Electrospun Fibrous Membranes And Kartogenin

Rotator cuff tears(RCT)are main challenging shoulder diseases which cause pain and disablility of shoulders.Arthroscopic rotator cuff repairs have been shown to have good long-term results.However,it is unavoidable that some patients still develop retears The main challenge of tendon-bone healing after rotator cuff repair is that the fibrocartilage zone is hard to regenerate in the enthesis.In this study,we fabricated a functional engineered scaffold for regenerating tendon-bone enthesis in RCTs by taking advantage of both the structural guiding ability of aligned nanofibers and the biology effects of KGN.ObjectiveTo evaluate the effect of Polycaprolactone(PCL)fibrous membranes with aligned nanofibers loaded with KGN on tendon-bone healingMethods and materialsPolycaprolactone(PCL)fibrous membranes with aligned nanofibers loaded with or without KGN were fabricated using electrospinning and characterized using scanning electron microscopy(SEM).The release of KGN from PCL membranes and the effects of KGN on proliferation and differentiation of mesenchymal stem cell were investigated.KGN-PCL scaffolds were applied to an acute rat RCT model and evaluated in vitro experiments.ResultsIn vitro,PCL scaffold prolonged the KGN duration of action and 100umo/L KGN-PCL promoted proliferation and chondrogenic differentiation of BMSCs.In vivo.KGN-PCL improved fibrocartilage formation,collagen organization and biomechanical properties.ConclusionPCL electrospun fibrous membranes with aligned nanofibers act as the KGN delivery system and enhance tendon-bone healing in RCT by promoting fibrocartilage formation and collagen organization and strengthen the healing enthesis.