The Level And Clinical Significance Of Interleukin-16 In Adult Primary Immune Thrombocytopenia

Objective:Adult primary immune thrombocytopenia(ITP)is an autoimmune mediated hemorrhagic disease.Interleukin-16(IL-16)directly affects cellular or humoral immunity by mediating cell crosstalk between T cells,B cells and dendritic cells.Some studies have focused on the role of IL-16 as an immunomodulatory cytokine in Thl polarization of autoimmune diseases.Methods:The expression of IL-16 in monocytes and NK cells was measured by flow cytometry;the mRNA expression levels of pro-il-16,caspase-3 and T-bet in ITP patients and controls were measured by real-time fluorescence quantitative PCR;the concentrations of IL-16 in plasma and bone marrow supernatant were measured by ELISA;rs11556218,rs4072111 was detected by time-of-flight mass spectrometry Single nucleotide polymorphisms.Results:The results showed that the expression levels of IL-16 in bone marrow supernatant and plasma were significantly increased in ITP patients and healthy controls.In addition,we also measured the expression of IL-16 after a single dose of high-dose dexamethasone for 4 days.The levels of IL-16 in bone marrow supernatant and plasma in active ITP patients were higher than those in healthy controls(P<0.05).At the same time,the mRNA expression of BMMCs(pro-il-16,caspase-3)and PBMCs(pro-il-16,caspase-3 and T-bet)in ITP patients were higher than those in healthy controls(P<0.05).After HD-DXM treatment,plasma IL-16 level and PBMCs gene expression(pro-il-16,caspase-3 and T-bet)decreased(P<0.05).In conclusion,the abnormal level of IL-16 plays an important role in the pathogenesis of ITP.Conclusion:HD-DXM combined with IL-16 may provide a new perspective for the immunomodulation of ITP.