Mechanism of glyceraldehyde-3-phosphate dehydrogenase - NAD(+) binding and product transfer

The fluorometric titration of GPD with NADThe method was used to study the effect of modification of the Cys-149 thiols on the GPD affinity for NADHow a metabolite (such as 1,3-BPG) transfer between pairs of enzyme catalyzing consecutive reactions (as GPD and PGK in glycolysis) is controversial. To further explore this situation the effect of PGK on the G3P oxidative phosphorolysis (or arsenorylsis), and on the relaxation of the equilibrium transposition was examined (by adding TIM to shift the equilibrium of GPD reaction toward the reverse direction). In contrast to the higher equilibrium level of NADH expected in the presence of PGK, the (NADH)...