Mechanisms of Clathrin-Mediated Endocytosis in Budding Yeast

Clathrin-mediated endocytosis (CME) is a major endocytic pathway that is highly conserved among eukaryotes. CME is critical to maintain cell homeostasis, attenuate receptor signaling, generate turnover of proteins at the plasma membrane (PM), and modulate lipid composition of the PM. In the budding yeast S. cerevisiae CME, cargo on the PM is captured, then invaginated into a tubule that pinches into a vesicle to travel into the cell interior. This process relies on the progressive recruitment and coordination of the activities for approximately 60 different proteins. Here, I describe three studies to investigate how the recruitment and activities of endocytic proteins are coordinated during various steps of endoctyosis to ensure successful endocytic events. (Abstract shortened by ProQuest.).