| With the rapid development of integrated circuits,the electrononic chip technology has approached to its limit,so it is imperative to research and develop new logic devices with new principle.Due to its high parallelism,large storage capacity and low energy consumption,molecular computer that can combine with optics,chemistry,electricity and biology has become a new type of interdisciplinary discipline and has been widely researched.DNA is a biomolecule that can construct logical models on the nanometer scale.The powerful complementary pairing function between the four bases of DNA makes DNA perform amazing information storage function.Based on DNA hybridization or DNA chain replacement reactions,large-scale and complex logical operations can be quickly realized.At the same time,based on the special structure of DNA and the interaction between DNA and nanomaterials,the logic operation systems that realize a variety of functions can be constructed.Nowadays,the biological computing based on DNA has gradually developed from simple logical operations to logical operations that can realize more complex functions,which provides more possibilities for biological computing.In this paper,based on the reacting platform between DNA/DNA and DNA/nanomaterial and combined with the characteristics of Fluorescence Resonance Energy Transfer,we realized the development of new optical logic devices using fluorescence method as a detection method and explored its application in the field of biosensors.This paper mainly completed the following work:1.Based on the special structure of DNA,the platform of the logical comparison system was constructed.Using DNA hybridization reaction and DNA replacement reaction and combined with fluorescence detection method,a variety of logical systems were constructed.Also,based on the reaction principle of the logical system,the selective detection of HIV and HCV was realized.In this work,DNA molecular beacon structures with fluorescence and quenched molecules at both ends and G4 structures rich in G bases were designed,and the reacting platform of a mathematical logic comparison system based on the two DNA structures was constructed.Based on the principle of complementary pairing of DNA bases and DNA chain replacement reaction,the DNA input sequences were encoded through the logic relationship between the input and output signals displayed in the truth table.Finally,based on the analysis of the output signals derived from the interaction between the input signals and the reaction platform,the fluorescence mathematical logic comparison systems of "2 input-3 output","3 input-3 output" and "4 input-3 output" were constructed successively,which realized the complex logic comparison functions,including ">","<" and "=".In addition,based on reaction principle of "2 input-3 output" logic circuit,a system based on "2 input-3 output" logic of DNA biosensor was constructed.HCV and HIV genes with high detection sensitivity was studied in the sensing system through analyzing the fluorescence output signal after adding HIV and HCV gene to the sensing system,and the detection limit of 500 f M.Also,the sensing system has good selectivity.2.Large-scale mathematical systems have been constructed based on the DNA/GO platform by encoding DNA hybridization reactions and the properties of Fluorescence Resonance Energy Transfer.Firstly,graphene oxide(GO)nanomaterials were prepared and non-selectively adsorbed single-stranded DNA sequences via ?-?bonds.Due to the effect of Fluorescence Resonance Energy Transfer,GO can quench the fluorescent signal modified on the DNA.Based on encoding the DNA input sequence and the reaction between the DNA inputs and GO/DNA platform,the "6-bit" square root and the "9-bit" cube root of mathematical logic operation devices is constructed through the normalization analysis for fluorescence output signals,which can implement the square-root mathematical calculation within 50 and the cube-root mathematical calculation with 500. |
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