Study On Pretreatment Of Public Safety Poisons And Construction Of Surface Enhanced Raman Spectroscopy Detection System

The proliferation of public safety poisons has infiltrated people’s production and life to varying degrees,posing great threats to public safety,people’s health and environmental protection,and bringing new challenges to the management and control of security departments.Most of the existing public safety poison detection methods are based on laboratory testing.Researchers are actively developing new methods on the basis of traditional analytical methods such as chromatography and mass spectrometry,and some combination methods have solved part of the detection requirements.These methods and strategies still have the problems of relying on expensive instruments and time-consuming analysis,and it is difficult to realize the demand for rapid detection of poisons on the spot.Therefore,it is of great significance to study fast,portable,and sensitive methods for rapid detection of poisons.In this paper,the surface enhanced Raman spectroscopy(SERS)method is used to study the interaction with the target,combined with the simple pre-treatment of typical poisons in different matrices,to solve the problem of effective enrichment and acquisition of target poisons in complex matrices.The SERS detection system corresponding to the four types of poisons has been established.The main research contents are as follows:(1)A gold nanorod(Au NRs)substrate coated with cetyltrimethylammonium bromide(CTAB)was designed and prepared,and a SERS chip with high affinity to succinylcholine chloride(SCC)was constructed.Based on the affinity between the long-chain alkane structure and the quaternary ammonium salt,combined with simple pre-treatment,the rapid detection of SCC is realized.This method can detect 2×10-8 mol/L SCC in plasma within 7 minutes.The relative standard deviation(RSD)of the spectral intensity at 716 cm-1 is 14.09%,which reflects high detection reproducibility.This system provides new ideas for solving on-site SCC poison identification and possible clinical applications.(2)For fentanyl poisons in urine,a SERS method for rapid detection and differentiation of fentanyl was established.Through pH adjustment,dehydration,and concentration,the interference of urine is avoided.Using ethyl acetate as the extractant,the detection of furan and acetylfentanyl in urine is realized.The detection is sensitive and stable,and can reach the nM level.Combined with principal component analysis(PCA),different fentanyls and matrices can be effectively distinguished and identified,which can provide a way to solve practical problems for the on-site identification and judgment of newly listed poisons.(3)Using a portable,sensitive and flexible SERS substrate,and adopting the design idea of integrating sample collection and detection,the rapid detection of paraquat(PQ)in blood and urine is realized.The flexible substrate prepared based on gold nano dumbbell(Au NDs)nanostructure units has good hydrophilicity,which is conducive to the acquisition of target molecules.The established pre-processing and detection methods can achieve sensitive and rapid detection of PQ in real blood and urine within 10 minutes,with detection limits of 15 and 10 n mol/L,respectively.This provides a simple solution for the on-site assessment of poisoning cases and the detection and identification of clinical acute poisoning.(4)A SERS sensor based on Meisenheimer reaction was designed and constructed to realize the detection of trace trinitrotoluene.The complex system formed is transformed from a non-resonant state to a resonant state,which promotes the detection sensitivity of the molecule,and the detection limit is 2.147×10-11 mol/L.At the same time,its structural analogs nitrophenol(NP),picric acid(PA),dinitrophenol(DNP)and dinitrotoluene(DNT)also have good selectivity.This type of sensor improves the antiinterference ability of nitroaromatic poison detection and expands the scope of application of SERS in the field of public safety.