| Objective: The first study used Jingui Baoxin Mixture to treat and evaluate patients with acute coronary syndrome,and to observe its clinical efficacy and safety;the second study was based on proteomics technology combined with bioinformatics to analyze the differences in protein expression in serum,explore its targets and pathways,and preliminarily explore the mechanism of Jingui Baoxin Mixture in the treatment of acute coronary syndrome.Methods: The first study adopted the design method of a randomized controlled clinical trial.From August to December 2021,72 patients in the Department of Cardiology,Affiliated Hospital of Changchun University of Traditional Chinese Medicine who met the admission and exclusion criteria were randomly selected for inclusion,and the patients were recruited according to the ratio of 1:1.They were randomly divided into the experimental group of 36 cases and the control group of 36 cases.The control group was given conventional western medicine treatment,and the experimental group was loaded with Jingui Baoxin Mixture(60ml/bottle)on the basis of the control group,20 ml each time,3times a day,and the course of treatment in both groups was 4 weeks.The probability of major adverse cardiovascular events after 4 weeks of treatment,the quantitative score of TCM symptoms,the efficacy of angina pectoris,the efficacy of electrocardiogram,inflammatory factors(hs-CRP),blood lipids(low-density lipoprotein,high-density lipoprotein,triglyceride,etc.)were compared between the two groups.Ester),Seattle Angina Questionnaire(SAQ),and SF-36 Health Questionnaire.SPSS 20.0 software was used for data analysis.In the second study,DIA proteomics technology was used to detect 10 patients with significant clinical effects from the first experimental group.Serum samples were used at the time of enrollment and after 4 weeks of treatment,and the blood samples were divided into con(before treatment)and treat(after treatment)two groups.First,use equal amount of combined samples as pool samples for HPRP fractionation,and conduct LC-MS/MS(tims TOF＿DDA mode)analysis to obtain the DDA library database,then screen the differential proteins of the two groups and combine bioinformatics analysis to analyze the differential proteins for further analysis and exploration.Results: The results of the first study showed: 1.The two groups were compared in terms of age,gender,body mass index(BMI),heart rate,blood pressure,smoking history,drinking history,hypertension history,diabetes history,and disease type(ST-segment elevation myocardial Infarction and non-ST segment elevation myocardial infarction,unstable angina pectoris and other basic data were statistically analyzed,and the results showed that P>0.05,there was no statistical difference,and they were comparable.2.The total effective rate of the experimental group was 97.14% higher than that of the control group,82.35%,in terms of the efficacy of angina pectoris between the two groups,P<0.01,and the difference was statistically significant.3.In terms of adverse cardiovascular events,the incidence rate of the experimental group was 11.42%,which was better than that of the control group,which was 35.24%,P<0.05,and the difference was statistically significant.4.In terms of quantitative scores of TCM syndromes,the scores of the two groups can be reduced when compared within the group,and the test group is better than the control group when compared between the groups,P<0.05,the difference is statistically significant.5.The total effective rate of the experimental group was 85.71% higher than that of the control group 76.47%,P<0.05,the difference was statistically significant.6.In terms of inflammatory factors(hs-CRP),the differences between groups were statistically significant,P<0.05.7.In terms of blood lipids(low-density lipoprotein,high-density lipoprotein,triglycerides),after 4 weeks of drug treatment,the low-density lipoprotein levels of the patients in the experimental group and the control group were lower than those before treatment,and the difference was statistically significant.Scientific significance P<0.05,high density lipoprotein and triglyceride P>0.05,there was no statistical difference;there was no statistical difference in blood lipid levels between groups P>0.05.8.Comparing the scores of the five dimensions of SAQ,the differences within and between groups were statistically significant,P<0.05.9.In terms of SF-36 health questionnaire,there are statistically significant differences in physiological function,physiological function,physical pain and mental health between the two groups,P<0.05,and other indicators P>0.05,there is no significant difference.10.No adverse reactions were found in both groups within 4 weeks of treatment.The results of the second study showed that a total of 3473 proteins were identified by DIA proteomics identification technology,and a total of 8 differential proteins with annotation function were screened,which were human immunoglobulin heavy chain variable regions 1-45(Immunoglobulin heavy variable region 1-45).1-45),Alpha-1-antitrypsin short transcript variant 1C4(Alpha-1-antitrypsin short transcript variant 1C4),extremely non-functional immunoglobulin heavy variable 7-81(Probable non-functional immunoglobulin heavy variable 7-81),ferroportin(Hephaestin),lysozyme(Lysozyme),IGL@protein(IGL@protein),proprotein convertase subtilisin/kexin type 9,complement C1 r subgroup samples Protein(Complement C1 r subcomponent-like protein).According to the results of bioinformatics analysis,the GO functional items of the differential proteins are mainly metabolic process,biological regulation,response to stimulation,cellular process,immune system process and other biological processes;binding,catalytic activity,molecular function regulator,extracellular domain part,Molecular and cellular functions such as extracellular regions,cell parts,etc.The enrichment analysis of the KEGG signaling pathway found that the identified differential proteins were mainly in the complement and coagulation cascades,mineral absorption,cholesterol metabolism and salivary secretion signaling pathways.(Salivary secretion)These four pathways were significantly enriched.Conclusion: The first research Jinguibaoxin mixture has a significant effect on patients with acute coronary syndrome,can relieve the symptoms of angina pectoris,improve the symptoms of traditional Chinese medicine and the quality of life of patients(SAQ,SF-36),inhibit inflammatory factors,reduce The probability of occurrence of blood lipids and adverse cardiovascular events,and the safety are also good.Therefore,the drug can be widely used in clinical practice.The second study Jinguibaoxin Mixture may have the effect of stabilizing atherosclerotic plaque,and its mechanism of action is related to the mineral absorption,complement and coagulation,cholesterol metabolism and salivary secretion pathways related to atherosclerosis. 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