A Study On The Related Factors Of Intestinal Flora Characteristics,Childhood Trauma And Others In Adolescents With Non-suicidal Self-injury

OBJECTIVE:To explore the risk factors or protective factors of Non-suicidal self-injury(NSSI),the characteristics of intestinal flora in NSSI adolescents,and the relationship between childhood trauma and intestinal flora characteristics,and to provide a possible theoretical basis for the prevention of NSSI in adolescents.METHODS:51 adolescents who met the diagnostic criteria for NSSI in the Diagnostic and Statistical Manual of Mental Disorders(DSM-5)were selected as the experimental group(KD).These adolescents were treated in the outpatient and inpatient departments of the psychiatric department of Guangdong Provincial People’s Hospital from November 2020 to November 2021.24 healthy volunteers were selected as the control group(NOR1).24 adolescents with depressive disorder without NSSI were included in control group 2(NOR2).In the KD group,childhood trauma was classified to subgroup KD1 and KD2.Psychological assessment was performed with the Childhood Trauma Questionnaire,the Adolescent Psychological Resilience Scale,the 24-item Hamilton Depression Scale,and the NSSI behavioral questionnaire.16SrDNA gene sequencing was used,and linear discriminant analysis was performed using LEfSe software.RESULTS:1.Most of the NSSI adolescents have experienced childhood trauma.Its incidence rate was 84.3%.The incidence of affective neglect and somatic neglect was 64.7%and 50.1%,respectively.There was a moderate positive correlation between childhood trauma and depressive mood and NSSI frequency(r-values were 0.495 and 0.466,respectively,all P<0.01,).Childhood trauma showed a moderate negative correlation with psychological resilience(r=-0.441,P<0.01),while childhood trauma was moderately positive associated with depressive mood(r=0.441,P<0.01).Childhood trauma significantly predicted the occurrence of NSSI(β=0.477,SE=0.095,P<0.001).Trauma negatively predicted psychological resilience levels in childhood(β=-0.844,SE=0.259,P<0.001).The interaction term of childhood trauma and depression was significant(β=-0.011,SE=0.004,P<0.001).NSSI(β=-0.128,SE=0.071,P<0.05).The interaction term of psychological resilience and depression mood was significant(β=-0.010,SE=0.001,P<0.001).2.There was a significant difference in Alpha diversity of gut microbiota between KD and NOR1 groups.The diversity of KD on shannon and simpson than NOR1(4.81 vs 5.21;0.02 vs 0.01,both P<0.05).The difference in the relative abundance of gut flora between KD and the other two controls is reflected at the class,order,family,genus,and species levels.Bacteroides were the dominant flora of the KD and NOR2 groups.Mitsuokella is the dominant flora that distinguishes KD from NOR2.3.There was no significant difference in the gut flora Alpha diversity between the KD1 and KD2 groups.However,there was significant relative abundance between the two groups.These differences are reflected at the order,family,and genus levels.The daunoryophyte-RF9 order is the dominant flora of the KD1 group.The proportion of bacterial levels in the Brucelidaceae and daunorophyte RF9 orders in the KD1 group was significantly higher than that in the KD2 group.The proportion of bacteria in Trichospira,Propionibacterium,and wart microfamily in the KD1 group was significantly lower than that in the KD2 group(all P<0.05).CONCLUSION:1.Emotional neglect,emotional abuse,physical neglect and physical abuse,and depressed mood are risk factors for developing NSSI in adolescents,while psychological resilience is protective factors.Childhood trauma can significantly affect the level of mental resilience during adolescence.The level of psychological resilience is a direct negative prediction of NSSI.Depression mood is a direct positive predictor of NSSI.Psychological resilience plays a partially mediating role in the relationship between childhood trauma and NSSI.Depression mood is an important regulator in this process.2.NSSI adolescents have poorer gut flora Alpha diversity than healthy adolescents.The relative abundance of gut flora in NSSI adolescents is changed at the class,order,family,genus,and species level,especially reflected in the increased intestinal inflammatory flora.3.There was no difference in Alpha diversity in the gut microbiota between NSSI adolescents with childhood trauma and those without childhood trauma.Partial inflammation-associated microflora is elevated in those with childhood trauma.