Study On Enzyme Assistant Extraction Of Procyanidins From Seedpod Of Lotus And Its Antioxidation In Oils

Procyanidins is a type of polyphenols compound, in recent years, much studies and agreat amount of work of procyanidins have made clear that procyanidins has many outstanding characteristics such as the ability of antioxidation,seavenging·OH, and excellent biological and pharmacologic activities.The study has used the seedpod of the lotus of Nelumbo nucifera Gaertn, try to enhance the extraction ratio of procyanidins through enzyme assistanted. And study the antioxidation of procyanidins in unsaturated fatty acid,oils and lipoxygenase.And add it to hams to research its anoxitantion in food which contain oils.Furthermore, used the isobologram to study the interaction of procyanidins with other antioxidant.My main research results are as follows:1.The cellulase and pectase have been used in the extraction process.An orthogonal experiment is carried out to optimize the parameters of enzyme time, temperature and so on. The best technic parameters are like this:the amount of cellulase is 0.7%, the amount of pectase is 0.1%, enzyme temperature is 55℃, enzyme time is 2.5 hours. Then combine the methods of microwave and ultrasonic, it find that compare with the method of ethanol extraction,the technic after optimized can increase the extraction ratio greatly.2.HPLC and IR have been used to compare the form of the samples, the result shows that enzyme assistant extraction can enhance the extraction ratio of procyanidins observably and the forms have no distinguish. On this basis I compared their antioxidation by DPPH and find that they have the same antioxidation.3.Extract lipoxygenase from bean, linoleic acid system has been used to study the inhibit effect of different concentration procyanidins and tea polyphenol to it. The result shows that procyanidins and tea polyphenol have the same inhibit effect to lipoxygenase in linoleic acid system. When the concentration is 0.08mg-mL-1,the inhibition ratios of procyanidins and tea polyphenol are 88.68% and 91.77% and the IC50 are 0.0470 mg·mL-1 and 0.0468 mg·mL-1.4. Study on the antioxidation of procyanidins in unsaturated fatty acid by oven. The result shows that:in oleic acid, no matter use procyanidins alone or with other antioxidants, they all showed excellent antioxidation, and the best is procyanidins compound with vitamin E.In linoleic acid and linolenic acid, procyanidins with different concentrations all has some antioxidation.5.Study on the antioxidation of procyanidins in oils by oven. The results show that procyanidins has favorable antioxidation in many kinds of oils. In tea oil, only a little procyanidins shows excellent effect. And when it compounds with vitamin E, the oxidative induction time of lard oil has been prolonged remarkable.6.Combine isobologram with DPPH, I studied the interaction of procyanidins with other antioxidants. The points of combinations were found to be lying below the additivity line and 95% confidence intervals in the Isobolographic graphs.There was a significant difference between the theoretical IC50,add values and the experimental IC50,mix values.The interaction index values of the combinations were less than 1.these results indicated that LSPC combined with VC,VE and cysteine had synergistic antioxidant properties.7.The best matching of procyanidins with VE in lard oil has been used in harm, and it shows outstanding antioxidation.