Construction And Characterization Of A Large Insert Deep Coverage BAC Library Of Rice PA64S

A genomic DNA library is a collection of recombinant DNA clones which contain random fragments of a certain organism. Bacterial artificial chromosome (BAC) library provides a powerful tool for physical mapping, positional cloning, genome comparative analysis, homologous gene cloning, research on gene expression and regulation etc. because of its large insert size, stability and easy manipulation.PA64S is the maternal cultivar of the super hybrid rice LYP9. Its genome research will help in comparative studies between and cloning of agriculturally important genes from the two parental genomes 9311 and PA64S, facilitating the genome study of LYP9.In this experiment, we have constructed a large insert deep coverage BAC library of rice PA64S. The library contains 98 384-well plates,37,632 clones with an average insert size of 138 kb, representing nearly 12-fold of the rice genome. The empty-vector rate is 1.5% and the contamination of mitochondrial and chloroplast DNA are 0.35% and 1.85% respectively.