Modelling Optimization For FTIR/ATR Spectroscopy Analysis Of HGB In Soluble Blood

Using Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) spectroscopy established a direc and rapid quantification method of analyzing human blood hemoglobin (HGB) without any chemical reagent. Optimized the wavebands of quantitative analysis by moving window partial least squares (MWPLS) model.113 samples of human soluble blood were collected and prepared, detected the spectroscopy and the HGB data of all samples were measured by BC-3000Plus automatic blood cell analyzer in the meantime, which were used for modeling and checking. All the samples were divided into model set (80 samples) and test set (33 samples), the model set included the calibration set (50 samples) and prediction set (30 samples). Which were used for modelling optimization for spectroscopy analysis.The optimal MWPLS model for HGB had a waveband of 1751-1570 (cm-1), the corresponding factors were 6, with its RMSEP,RP to be 4.645g/L,0.941 respectively. Which was obviously superior to the direct PLS model of the whole spectrum, and had few wavenumbers, that greatly reduced the complexity of the model. Then retested the models after all the procedures, got the RMSEP and RP to be 5.098 (g/L),0.934 respectively. The correlation of the prediction was very well, and then established high accuracy anlysis modle for HGB in soluble blood samples by FTIR/ATR Spectroscopy.