Seasonal Changes Of Bacteria In Beiluhe Aera Permafrost From Tibet Plateau

Earth's ecosystems has large areas of permafrost in the high-latitude and high-altitude areas in presence.More and more research shows that a lot of micro-organisms in the low-temperature of permafrost.These micro-organisms has a unique characteristics and physiologica to adapt low temperature, they has an important position and role in this particular ecosystem.We collected four different ecological sites of BeiLuhe permafrost area of Tibet Plateau:the edge of a small stream, the edge of frost moundthermokarst lake, the degradation of local vegetation, peaty soil of pingo were analyzed by fluorescence microscopy count, microbial culture and 16SrDNA-PCR technology, BIOLOG technique.The main results were as follows:1.Cultured bacteria in permafrost was significantly negatively correlated with pH.The total water content was significantly negatively correlated with micro-organi-sms and the pH.2.In same season cultured bacterial diversity was different in the four different sites of BeiLuhe permafrost area, different permafrost sites had different seasonal variation of cultured bacteria diversity.3.Seasonal changes of the microbial carbon utilization was not significant in permafrost of the edge of frost moundthermokarst lake by BIOLOG experiment.The microbial carbon utilization in the edge permafrost of small stream showed significant seasonal changes, and higher utilization rate.The microbial carbon utilization variations in the subsurface soil of the local degenerated vegetation were similar in May and August, the microbial carbon utilizationwas lower in November.The microbial carbon utilization rate of peaty soil of pingo was the highest in permafrost of Beiluhe, and the seasonal changes was significant.