Purification Of Style Glycoproteins Associated With Gametophytic Self-incompatibility And Studies On The Organ-specific Proteins In Citrus Grandis Var.shatinyu Hort.

S~es of do-us grardic r.shatitry~i Hoit ~e choosed as experhnattal material, which wew in only three days tlmgh artificial pollination of their own. About b2 style tisst~ ~ cut, and iriturated as homogeneity. 35% protein soluticm was gained by use of abstraction mid salting out. The solution showed nine protein brands through PAGE, but it was ob~iow that there was two dominative protein brands near positive anode. The solution showed only one protein brand (S~ peak) through ConA-Sepharose 4B affinitive chromotography column, which ~ one of die two exceptional proteins. We did experiments in order to determine die S~ peak protein's part biochemical natures. The results: S~ peak protein ~ basic glycoprotein,sugar accouting fcr 924~and it ~ made ~ of two subunits through SDS-PAGE. whose molecular weight were 38.0k!). 32.OkD and iso-electric point 72. 6.9. In addition, S~ peak protein's biochemical activity was experimented S~ protein could restrain the gro~1h of pollen tubes which germinated in vitro. The restrainarrt role was stronger v?th more S~ protein in certain extent We took for Yeast's rRNA as substrate to determine S~ protein's nuclease activity which was 11 8.3u/mg.The final fact was diat S~ protein could degtadate RNA strongly. Above experimental resuhs coincided v~idi reported S-glycoproteins associated whh selfirrcompan'oility. After we enough understood Citrus grc~iis Var.shaiinyu Ho~t gametophytic self incompatibility smicttne and biochemical bases did we begin doing experimentSo we could deduce exactly diat die S~ glycopn)tein we ptnified is S-protein associated ~viui Citrus gram~lis \~shc~j I-bit gametophytic self-incompatibility From what we had done,we dieussed minutely S~ protein's important role dtning the reaction of Citrus grandis Var.shatinyu Hort gametophytic self incompanl,ilitymar was, afcer artificial pollination to Citrus grcn~dic Var.shat~i Hort in three days, S-protein of style was translated and expressed in magnitxle. In the same time, pollen tubes of germinated pollen were grov~ing foreheacL But S-protein could enter speflcally into pollen tubes and degrariate its rRNA. Finally, pollen tubes stoped growing because its proteins were not expressed,.and die reaction of Citrus grandis Var.shatfrzyu Hort gametophytic selfincompaffbility occineiMorever, Soluble proteins were analyzed by use of the techr4e of SDS-PAGE, which were extracted fiom various organs (stem. leafs sepia]. petaL stamen.. style. ovaiy) of Citrus ra'idis \~rShaz~i Hort.The majorities of the proteins were expressed in these organs. But there are some proteins specific to one organ,They were 31.0W in stem,56.OkD in leaf,59.OkD, 58.OkD.. 44.OkD..37.0W.36.0W. 35.5k!). 33.0k!), 21.0k!), 15.4k!) in stamen,43.OkD, 38.5k!) in style,33.SkD in ovaxylEF-SDS two-dimensional electrophoresis showed the total proteins of die stem. leaf.stamen. style. Many 51)5-PAGE proteins bands represented several proteins dots in LEF-SDS-PAGE.Proteins ananvsis of the various organs showed that stamen contained the gtealest amount and septa] the least amount...