| Gene is the basic life unit that controls the genetic properties of organisms. One of the most important jobs is to identify all transeriptable genes in the genome following the whole genome sequencing. Scientists have been trying to establish various gene-rapping systems, especially functional gene-rapping systems, for novel gene finding and studying. And now, a lot of systems of this kind have been established according to different research aims and approaches: for example, differential display techniques, yeast twoybrid system and so on, this directly led to the cloning and studying of a lot, or maybe most of the known genes. Protein is the executor of life. As we know, after being synthesized in cell plasma, proteins must be transported to particular subellular compartments to carry out their own tasks, we call this process rotein sorting'. Protein sorting process depends on the ip code? particular amino acid sequence located in the protein. For instance, protein secreting is directed by the leader peptide at the Nerminal of the protein. According to this characteristic of protein, several effective gene cloning systems had been established, such as signal sequence trap system, nuclear localization signal trap system and the forth. Since the genes cloned by these methods encode proteins with particular protein sorting signal, we name this kind of gene cloning method signal trap. In this research, suc2 signal sequence trap system was established and a mouse embryonic eDNA library was screened with it. The work is divided into five parts as following: 1) Cloning suc2 gene; 2) Targeting mutation of genomic suc2 gene of yeast EGY48; 3) Constructing suc2 eukaryotic expression vector for library screening; 4) Testing the efficacy of established system; 5) Screening E. 11 mouse eDNA library with the system. A conclusion can be drawn from the results that the system established can give an effective and efficient library screening for signal peptides.
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