Preparation And ELISA Of Monoclonal Antibodies Against Streptomycin

Our research aimed to find a better means to detect the strepomycin residue in food.The antigen was synthesized by using a strepomycin-oxime derivative coupled to bovine serum albumin ,then injected to Balb/c mouse. A cell line ,3B1-G12-C12,which secretes monoclonal antibodies against streptomycin stably was abtained by the technology of hybridoma.The antibody characterized by IgGl isotype and the only detectable cross-reactivity was shown to be with dihydrostreptomycin of about 120%. The hybridoma cells line which average number of chromosome 96 steadily secretes antibodies after subcultured 15 times. The ELISA liters of cells supernate and ascites were exceeded 1∶ 1.0×104 and 1∶ 1.0×108 seperately.An indirect competitive ELISA was founded base on the monoclonal antibody.It's detectinon limit for streptomycin was 2.0 ng/ml. Recovery range in milk and urine is from 90% to 105%.We can say ELISA is a better means to detet the streptomycin residue in food by comparing limit, efficiency, defmition,fee,et al. with other means.