Photosynthetic Bacteria's(PSB) Identification And The Study Of Immune Function And Growth Performance

Four photosynthetic bacteria (PSB) strains were isolated, purified and identified in the study. The effects of them on mice's immune function and carp's growth performance were studied. The results were as follows:1.36 PSB strains enriched from 19 samples. 5 strains were isolated, purified and identified. They were Wst22, Sc21, Sc42, Sx51 and Sx52. Wst22 and Sc42 were identified as Rp. palastris, Sc21 and Sx51 were identified as Rp. vannielii, Sx52 was identified as Rp. capsulate. The four PSB strains, Wst22 Sc21 Sc42 Sx51, grow rapidly and can be conserved for a long time.2. The resistant function to pathogenic E. coli and Salmonella enteritidis of four PSB strains was test that they had different function by Oxford cup test. The diameter of the antimicrobial circle could reach 30~32mm in the test of E. coli by Wst22, and the diameter of the antimicrobial circle could reach 20mm in the test of Salmonella enteritidis by Sc42. The others had the different resistance on the pathogenic bacteria.3. The results of safety check showed that four PSB strains didn't have toxic and side effect significantly on mice. Anatomic test showed there were no significant difference in the splanchnic organ structure between treatment and control.4. The results of mice immune function test indicated that four PSB strains could promote the development of mice immune function organs. The spleen index and thymus index of the treatment were higher than control. Thymus index of the mice treat with Sc42 was significant from control (p<0.05). Spleen index treated with Sx51 was significant with control (p<0.05). Four PSB strains could enhance the phage index of mice peritoneal macrophages significantly (p<0.05). The results indicated that four strains could enhance mice's immune function, especially the function of Sc42 and Sc51 were significant.5. The results of carp's growth performance indicated: The mixed nutrient solution of PSBcould improve the increase of carp's weight than other three groups (carp's diet of Juxing, flavomycin, "Yixubao" ), they are 9.5%, 9.6%, 15.4% respectively. And it could decrease the feed consumed/gain of carps than others, they are 2.6%, 6.3%, 4.8% respectively. All those showed that the mixed nutrient solution of PSB could enhance the growth performance of carp.From all the above, it can be concluded that the four PSB strains can accord with the standard of the strain of probiotics not only on the living condition and resistance of the pathogenic bacteria, but on the immune function and growth performance of animals.