| Resveratrol (trans-3,4'-5-trihydroxystilbene) is a naturally occurring phenolic compound of the stilbene family. It does not have a wide distribution in the plant kingdom and the resveratrol story does not begin with its recent discovery in red wine. New roles of resveratrol as a phytochemical of human health benefits are revealed as its association with reduced cardiovascular disease and reduced cancer risk has been demonstrated. Resveratrol has been investigated both for its biological role in plant defense against pathogens and for its pharmacological properties.Resveratrol synthase (RS; EC 2.3.1.95), a key enzyme in biosynthesis of stilbene-type phytoalexins, catalyzes the formation of resveratrol from one molecule of coumaroyl-CoA and three molecules of malony-CoA. Resveratrol synthase is present only in the plant species where resveratrol is found. Much attention has been focused on resveratrol and RS gene because of their implication as an important part of the plant defense system.In this study, the complete cDNA of resveratrol synthase was cloned from peanut by RT-PCR. Two plant constitutive expression vectors under the control of CaMV35S promoter were constructed by inserting the RScDNA into pCAMBIA1301-pT Ω 4A. The RS gene was introduced to tobacco by Agrobacterium-mediated transfer method, and 30 individual transgenic plants have been obtained. Results of PCR and Southern blot analysis showed that the foreign RS gene has been integrated into the tobacco genome in all the transformants. The transcription of the foreign gene in the transformants was also clarified by Northern blot analysis.
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