Isolation And Identification Of Glyphosate-Resisting Or Glyphosate-Degrading Microbe And Studies On Biological Quality

Eleven strains of glyphosate-resisting bacteria and thirty-seven strains of glyphosate-degrading fungi isolated from soil on which the glyphosate herbicide Baicaoqing or Roundup had been applied and from soil in a glyphosate factory. None of the eleven pure cultures of glyphosate-resisting bacteria can utilize glyphosate, but all of these strains have different tolerance to glyphosate. One of these strains, A10, still showed tolerance when the density of sodium glyphosate was up to 7g/l, and it was identified as Bacillus brevis according to the structure of cells, biochemistry and physiology characteristics and G+C% concentration.It was found that the strain, Tl, has the best ability to degrade the phosphonate herbicide glyphosate when we determined the ability of the thirty-seven strains of glyphosate-degrading fungi by HPLC. It showed 53.9% glyphosate degradation in 7d in sodium glyphosate medium and 69.4% glyphosate degradation in 7d in Roundup medium. After adjusted culture medium and culture condition, it showed 86.2% glyphosate degradation. Tl was identified as Paecilomyces varioti Bainier according to the configuration of mycelium, spores and lawn.The mutation strain, Tla, was obtained when Tl was disposed by ultraviolet radiation, which tested was hardly able to grow on sodium glyphosate as the only phosphorus source, and tested could not utilize sodium glyhosate by HPLC. But Tl still showed resistance to sodium glyphosate.