| The mammiferous testis perform important functions of producing sperm and secreting male hormone. The capillary vessel of testis which is a part of Blood-Testis Barrier transports oxygen, hormone and nutriment in order to maintain normal function. Microcirculation is the blood circle from arterile to venule. Blood-Testis Barrier is the harrier between capillary vessel and seminiferous tubule which is important to produce sperm and maintain male procreation function. At present, The research limit in pathology and clinic of adult human or animal and need more study on developing and aging animal. In our experiments, the morphological alterations of testis microvascular architecture, eNOS distribution and Blood-Testis Barrier at different ages had been observed by microvascular corrosion casting technique, SEM, TEM, immunohistochemical method and morphometry. These results were not only theoretically important but also practically valuable in physiology, pathology, aging biology, morphology and so on.The experiment materials were SD rats. According to their developmental characteristics, we selected six groups of rats: postnatal 1 day, postnatal 3 weeks, postnatal 3 months, postnatal 12 months, postnatal 18 months and postnatal 24 months.Each group included 30 rats.The results of the experiments were analyzed with the software of STAT on computer.The results were as follows:l.The microvascular distribution : The capillary vessel of rat testis were distributed among seminiferous tubules. The capillary vessel were distributed regularly in testis from postnatal I day to postnatal 12 months, and loosely in postnatal 18 months rat, and irregularly in postnatal 24 months rat. The capillary vessel number in every unit area increased and the microvascular wall incrassated gradually with aging.The number ratio between capillary vessels and seminiferous tubules were on the trend of increase from 3 weeks to 3 months and decrease from 3 months to 12 months.2. Testicular microvascular architecture of adult rat: Testicular arteries emitedparrelling branchs which became arterile and formed twist bends into testis parenchyma. Then the arterile eminted intertubular capillaries and peritubular capillaries forming rope-ladder-like pattems.Then the venule were collected and became testis veins, then mass into rete venosum, finally some venule formed pampiniform plexus and others entered into varicosity.3.Changes or Testicular microvascular architecture: Testicular capillary vessel formed intertubular capillaries and peritubular capillaries.but donot formed rope-ladder-like patterns capillary net in postnatal 3 weeks. There were rope- ladder-like patterns capillary nets around seminiferous tubules in postnatal 3 and 12 months. Testicular microvascular architecture became irregularly in postnatal 18 and 24 months with testis atrophing.The diameter of capillary vessel increased with aging. The diameter of arterile was less than that of venule.4.Influence of NO on microvascular permeability: Specimens from postnatal 1 day showed little immunoreactivity for eNOS. In contrast, blood vessel endothelium and Leydig cells and spermatids in postnatal 3 weeks testes were strongly expressed eNOS.The number of positive blood vessel endothelium, Leydig cells and spermatids in postnatal 3 months was conspicuously increased. In postnatal 12 and ISmonths testes, the number of postive cells persistently increased.In 24 months testes, some spermatocytes also displayed strong eNOS immunoreactivity. From 3 weeks to 3 months, the permeability of microvascular was on the trend of increase while it was on the trend of decrease from 12 months to 24 months.S.BIood-Testis Barrier: The basal lamina of endothelium cells developed from thin, broken and unequal electronic density to thick, full and high electronic density with aging. The number of plasmalemmal vesicle increased from young to adult and decreased gradually in old age. The basal lamina of seminiferous tubules thickened gradually with aginy. . There uas not tight junction betwee...
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