Protoplast Fusion Between Geotrichum Candidium2.498 And Phanerochaete Chrysosporium5.776

In this paper, we used the useful means in microorganism breeding in recent years which was called protoplast fusion, we used PEG 6000 as a reapent in the process of protoplast fusion of geotrichum candidium and planerochaete chrysosporium grown in the logarithmic metaphase of growth. In the meanwhile, the resistance of action of drugs and the destruction of a single parent were also used in order to select out the crasis which could decompose lignin in straw cellular effectively and also produce excellent SCP in the selecting cultural medium. In this paper, we mainly discuss all kinds of factors which affect the formation and regeneration, of phanerochaete chrysosporium and geotrichum candidium, and use various kinds of measures to identify the crasis. Specitic measures and research results were showed as follows:1. We measured the growth curve of geotichum candidium and phanerochaete chrysosporium by means of caculating the colong in plants and weighing the dry hypha respectively. The results showed that the logarithmic metaphase of growth of the twofurgus were 35 hours and 70 hours respectively.2. choosing 11 kinds of drugs to measure the resistence to dction of drugs of geotrichum candidium and planerochaete chrysosporium, at last choosing actidione acting as the medicine to identify the resistence3. Discussing all kinds of factors which affect the formation rate and regeneration rate of geotrichum candidium and phanerochaete chrysosporium by means of one-way experiment. The results showed that: the age of the fungus was the logarithmic metaphase of growth.4. Discussing the prime level combination of all kinds of factors which affect the formation rate and regeneration rate by means of intercross experiment.5. Choosing the specific depressor of mercapto-iodoacetamide (IAM) to destroy the geotrichum candidium of protoplast.6. using PEG6000 acting as reapent to help fuse at 35# in water bath getting 72 individual plants erases on selecting culture medium and make them exist from the first generation to the 10th generation, getting 12 indiviolual plants of steady erases.7. Compaing the appearance of colony, micrososmic configuration, antagonistic experiment of the parent and crasis to identity the crasis preliminarily8. Identifying the crasis further by means of full-cell solubility protein electrophoresis and isoenzyme to get 3 indiviolual plants of steady genetic erases.