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The Primary Study On Micromorphology Of Leaf And Chemical Component Of Syzygium Grijsii (Hance) Merr.et Perry

Posted on:2005-05-24Degree:MasterType:Thesis
Country:ChinaCandidate:X F LiuFull Text:PDF
GTID:2120360122997596Subject:Botany
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Syzygium grijsii (Hance) Merr.et Perry is a evergreen shrub which is distributed in Fujian, Guangdong, Guangxi province,ect.in China. Syzygium grijsii bears white flowers from June to August. Its fruits are in black and edibe. Presently, there has none of report about components from the front of Syzygium grijsii. The paper studies the shape of leaves and the microstructure of Syzygium grijsii from different populations by means of optic microscope and scanning electron microscope. Volatile oil and flavonoids in the leaf, nutrition components of the fruit, fatty acids of seed oil, red pigment of the pulp are studied by much chromatographic technique at the same time. The main results are as follows:1.There has distinct diversity of the shape of the leaves which are from 16 different populations in Fujian province. The leaves from Changtin county population, Mingxi county population and Liancheng county population, which shape is more roundisher and more wider. The proportion of the length and the width is about 1.5:1, so the shape of the leaves is broad ellepse; And the shape of the leaves from another populations is long ellepse, which the proportion of the length and the width is from 3:1 to 5:1.Micromorphological characters of leaf epidermis of different populations of Syzygium grijsii are examined by LM and SEM. There has no epidermal hairs in upper and lower leaf epidermis, and stomatal apparatus are only located in the lower's. Epidermal cells are atactic which pattern of anticlilnal wall have many types like sinuate, sinuolate, straight and bow in different populations, for example.The inner margin of the outer stomatal rim, the surface of the grard cell and the cuticular membrane are nearly smooth. The stomatal apparatus's size (the average size is 13.99X 10.70um), density (from 640 unit per mm2 to 1600 unit per mm2), exponent (from 12.44 to 28.51 ) and the adjuncts of the leaf epidermis are so discrepant in different populations that these characters have not taxonomic significance.2. There are many oil glands in the leaf of Syzygium grijsii. Collect the fresh tressesfrom different areas and dry in the shade and comminute them, then collect the volatile oil of the leaf by water distillation. The content of volatile oil in one year from different areas is from 0.40mL/100g to 0.52mL/100g.Volatile oil were studied by means of the GC/MS/DS and the content of each constituent was determined by area normalization method. The major components are sesquiterpenoid and terpenol. Thirty five constituents were identified which account for 84.09% of the volatile oil from Minhou county population, like P -Cubebene (16.31%), P -Cadinene (10.78%),Elixene (8.36%), - Cadinene (5.23%),Humulene (4.99%), -Muurolene (4.87%), P -Caryophyllene (2.74%), B -Elemene (2.73%), a -Cubebene (2.59%), Copaene (2.40%), P -Bourbonene (2.10%), ect. And thirty two constituents were identified which account for 72.08% of the volatile oil from Changtin county population, like Spathulenol (11.29%), B-Selinene (8.72%), - Cadinene (8.23%), -Muurolene (6.68%), Aromadendrene (6.24%), a -Gurjunene (5.58%), B-Elemene (3.58%), Humulene (2.14%), Elixene (2.01%), etc.3. On the base of preliminary experiment, flavonoids was found in leaf of Syzygium grijsii. The total flavonoids from different populations extracted and purified by polycaprolactan and determined. It is revealed that the content of flavonoids is the highest from June to August, it's 2.97% of Minhou population and 2.51% of Changtin population respectively. The content of Rutin and Quercetin in leaf of Syzygium grijsii from Minhou population were determined by HPLC. The results show that the content of Rutin is 0.611% and the content of Quercetin is 0.004%.4.The nutrition components of the dry fruit of Syzygium grijsii are studied by chemical methods. The compostions are water 73.29%, total sugar 3.27%, reducing sugar 2.27%, NaCI 0.58%, ash 3.25%, crude fiber 7.11%, crude protein 4.83%. Seventeen kinds of amino acids are found in pulp and seed. The content of amino acids in pul...
Keywords/Search Tags:Syzygium grijsii (Hance) Merr.et Perry, leaf shape, leaf epidermis character, volatile oil, flavonoids, nutrition components of the fruit, seed oil, red pigment
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