| Objective Phospholipase C- γ 1(PLC- γ 1) is rapidly activated in response to growth factor stimulation by a mechanism that relies on tyrosine phosphorylation, and catalyzes the hydrolysis of phosphatidylinositol 4, 5-bisphoaphate to inositol 1, 4, 5-trisphosphate and diacylglycerol. These second messengers mediate intracellular Ca2+ release and the activation of protein kinase (PKC), repectively. This crosstalkting pathway constitutes the cornerstone of a transmembrane signal transduction mechanism that is now known to regulate cell cycle, cell transformation, development, and apoptosis of cell. Although some studies about the mRNA expression and protein expression of PLC- Y 1 in different animals have been made, PLCG1 mRNA expression of the same animal in the different stages of development is not yet been reported, Thus, we investigated the role of PLC- Y 1 during the development by using SD rat as models through a total analysis of PLCG1 mRNA expression by RT-PCR(reverse transcriptase polymerase chain reaction).On the other hand, evidence has been presented for the existence of forms generated by alternative splicing for several of the PLC isoenzymes through standard molecular biology techniques. Human PLC- Y 1 has been reported in Locuslink existing alternative RNA splicing and produced two isoforms, PLC- Y la (NM002660, NP002651) and PLC- γ Ib (NM182811, NP877963) while there is only one RNA splicing forms in rat PLC- γ 1 (NM013187, NP037319). To determine whether there is existence of the same splice variants of rat PLC- Y 1 as human and explore it's possible significance, we carried out a series of analysis on the cDNA of rat PLC- Y 1 by using RT-PCR and sequencing.Methods and results The present study was divided into 2 parts according to individual purpose. 1. PLCG1 dynamic mRNA expression patterns of rat embryo, postnataearly rat, and adult rat Semi-quantitative reverse transcription polymerase chain reaction was used to detect the expression of PLCG1 mRNA in 44 samples extracted from 4 kinds of rat organs, liver, lung, kidney and brain in 3 different embryonic times, 7 different postnatal early times, and adult time. The results showed that PLCG1 was expressed in liver, lung, kidney and brain during 11 periods of growth and development of rat. Except the pregnant 12-day rat, in the other 10 periods the difference of PLCG1 expression was statistically significant in these 4 tissues. The PLCG1 mRNA expression level was most high in 7-day postnatal rat brain while lowest in 1-day postnatal rat liver.2. The examine of splicing forms of rat PLC- Y1 pre-mRNA According to the sequence of human PLC- Y 1 splice variant, the rat special primer was designed and synthesized. The rat RNA was reversed to cDNA, then the cDNA was amplified by the special primer and PCR products were sequenced and analyzed by using BLAST and some bio-informatics methods. We examined total 21 samples extracted from 6 kinds of rat organs, heart, liver, lung, kidney, eyeball, and brain in 3 different embryo times, 7 different postnatal early times, and adult time. The result did not show that rat PLC- Y 1 have the same splice variant (PLC- Y la, NM002660) as human for the present 21 samples. Conclusions1. PLCG1 was expressed in liver, lung, kidney and brain during 11 periods of growth and development of rat, while in the same 11 periods the expression of PLCG1 was various in these 4 tissues. These results also showed that expression of PLCG1 was in a tissue and development dependent manner. The differential expression of PLCG1 in various tissues and development periods suggested that PLCG1 was involved in the cell proliferation and differentiation during the growth and development of rat.2. There was no discovery that the same splice variant of PLC- Y 1 in rat existed as in human for the examined 21 samples. This result suggested that: A. These samples were not enough. B. When both of splice variant wereexpressed in the same tissue at the same time, the higher expressed splice form was amplified first and resul...
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