Studies On Fungistasi, ITS Sequences And Micromorphology Of Euphorbia In Anhui Province

The progress of researches was summarized in Euphorbia which mainly involved plant chemistry and pharmacognosy and so on. The paper had studied six species of Euphorbia in Anhui province and the results were as following: Firstly, The antifungal activities of six species acetone extracts were tested with Gibberella zeae,Sclerotinia scleotiorum,Verticillium dahliae,Glomerella cingulata,Mycosphaerella melonis. The results showed that six species extracts had strong antifungal activities. They were worth making further research,especially, roots of E.pekinensis and E.ebracteolata had more than 60% inhibiting rates against the hypha growth, and the latter had relatively stronger fungistasis. Secondly, By determining ITS sequences of five species, the result showed that: 1) the sequences of ITS1 ranged from 255bp to 264bp and those of ITS2 from 214bp to 236bp; 2) G+C content of ITS1 varied from 51% to 64% and that of ITS2 from 56% to 68%; 3) the five species was divided into two clades with the one including E.pekinensis, E.ebracteolata and E.esula whose bootstrap value was 98%, and the other including E.humifusa and E.supine which strongly supported by the bootstrap value of 100%; 4) ITS sequences may be useful for resolving phylogenetic relationships among species. Thirdly, By using paraffin sectioning to study the structure of leaf blades and investigating the leaf epidermis under light microscopy (LM) and scanning electron microscopy (SEM), the results showed that the structure of leaf blades and the characters of leaf epidermis were different among species. Therefore, all of the differences in the shape, size and array of epidermal cells, epidermal hair, the distributive position and number of stomatal apparatus, outer stomatalledge, inner margin of outer stomatal ledge, cuticular membrane can be regarded as the proof to differentiate Euphorbia . The purpose of above researches was to provide the molecular and micromorphological evidences for phylogenetic relationships, biology pesticide and distinguish the species from Euphorbia in Anhui.