Study On Cattle Preantral Follicle Culture In Vitro

In order to resolve the sources of preantral follicular oocytes, two methods of isolation were developed in our experiment., The mechanical method resulted in 12.6+3.6 preantral follicles per ovary, The average number of preantral follicles derived from mechanical method was significant lower than that from enzyme associated mechanical method. (p<0.05).This experiment choice the best basic culture system was DMEM+5.00mg/L Insulin+5.00mg/L Transherrin+5.00mg/L Seleniom+2.00mg/L cAMP+1.50mmol/L Glutamone+2.00mmol/L polylysosine+2.00mmol/L Hypoxanthin+5%SerumoExperiment compare to the influence of different culture methods on the preantral follicle cultured in vitro. The result indicates: after 6days cultured, the increments of oocyte in diameter were 29.8 and 32.0μm ,there were no significant differences (p>0.05),but the survival rate from polylysosine layer method no significantly differences than that from ordinary culture method(p<0.05).Experiment add to the different dose EGF and IGF—1 to the basic culture system from to the to cultured the cattle preantral follicles in vitro, the result indicates: EGF and IGF—1 can promote growth and survive of the preantral follicles.Experiment add to the different dose FSH and 17β-E₂ to the basic culture system to culture the cattle preantral follicles in vitro , the result indicates: FSH and 17β-E₂ can promote the preantral follicle growth and survival. When add to 0. 25mg/L FSH and 1.00mg/L, the result is the best .Experiment search the development after the preantral follicles were cultured for 12 days, the results indicate: after 12 days culture, the mean increments of oocyte in diameter were 62.84μm, there into , 18.76% can form the antrum.