Effects Of Te(IV) Stress On The Growth Of Spiurlina And The Spectra Studies

The biological effects of Te(â…£) stress on the growth of Spirulina and spectra characteristic were studied. Then the toxicity of Te (â…£) on spirulina and the bioaccumulation by Spirulina were studied in this paper. Some important results are as follows:First of all, adding Te(â…£) in the Spirulina at the growing phases, the last cumulating Te(â…£) concentration was the same. The effects of Te(â…£) stress on the growth of the S. platensis and S.maxima were studied. It was indicated that the stress of Te(â…£) were different on the growth of the two kinds of algae. The results were explained by the kinetic equation of alga and the stress intensity of Te(â…£).Secondly, the biological effects of Te(â…£) stress on the growth of S.maxima were studied. It indicated that the growth of S.maxima had been inhibited under high Te(â…£) stress. The pigments and protein of Spirulina decreased dramatically under Te(â…£) stress, and the relative activity of SOD was enhanced , then decreased under Te(â…£) stress. The exopolysaccharide can be accumulated more under Te(â…£) stress.Thirdly, from the studies of spectra, there were distinct differences between the control and frozed cells. The absorption spectra showed that the typical absorption peak was from phycobilisome after frozen up. And the photosynthesis energy transfer was depressed under Te(â…£) stress.Fourthly, there were great differences in these absorbance spectra between surviving cells and dried cells. The peaks of absorption had renewed after the dried cells re-absorbed. Whereas the PSI of dried cells suspended in water solution was halted after 14 hours, thus phycocyanim was excited by the energy that back transfer of the excitation energy in the PS I.Fifthly, after re-absorbing cells were laid on different intensity of light, it was concluded that the pigments in Spirulina decomposed, and the order is carotenoid, phycocyanim, chlorophyll, in turn. The intensity with time lengthening would decrease, especially in light.Lastly, the trace tellurium of Te-enriched Spirulina was determinated by ICP-AES. The Te contents were significantly higher than Se contents of Se-enrichedSpirulina. The amino acids of Te-enriched Spirulina were analyzed by HPLC, and the results showed the component was similar with control.