Heterogeneous Reconstruction Of Human Somatic And Sheep Oocyte And Isolation And Culture Of Like-Embryonic Stem Cell

Human Fetal skin fibroblasts were dissociated and cultured by explant tissue culture in vitro; Human fetal fibroblast cell had more normal chromosome content after at least twenty passages(2n=46); Compared with fluorescence method and blindfold method, the blindfold method was more efficient in the cleavage rate (48.83%vs 58.18) and producing reconstituted morula (73.81%vs 87.5% ).The nuclear of donor was injected into exnuclear occytes by electric fusion method and intracytoplasmic nuclear injection (ICNI), Nuclear transplantation units(NTs) were cultured in vitro . Although there was no difference in the cleavage rate (P>0.05) and the rate of producing reconstituted morula (P>0.05).Three voltages were entered into computer. It was more efficient in fuse the reconstituted embryos when the voltages were 1.6 KV/cm and 1.7KV/cm (57.69%,56.52%vs 41.58%).But there were not more efficient in producing morulas when the voltage were 1.6KV/cm (40%vs 52.38%,53.85%).The gather of like Embryonic Stem cell was got and cultured from the morulas. AKP (alkaline phosphatase) dyed the clong, It was positived.