| At present, in the field of floral genetic engineering,blue flowers have been the key factor in the study because of its graceful color and exiguity. The accumulation of anthocyanidin which derived from delphinidin is one of three prerequisites for breeding blue flowers. So the transformation with the key genes encoding delphinidin into non-blue flowers makes it possible to select blue flower cultivous.Flavonoid 3,5—hydroxylase(F3'5'H) is considered as a key enzyme in the synthesis delphinidin. Most natural flowers display blue color because of FS'5'H gene expression. This research design specially primers according to the sequence of F3'5'H obtained from Genbank, and a specific band of 1 500 bp was amplified from Pericallis cruenta's cDNA through PCR. Then it was ligated into pMD 18-T vector and analyzed by colony PCR and the enzyme digestion. Sequengcing and BLAST analysis confirmed the clone of F3'5'H gene.Chalcone synthase(CHS)gene is the key gene during the pathway of synthesizing anthocyanidin .And expresses specifically in the petal. DNA was isolated from Pericallis cruenta with modified SDS method. Specific primers were designed according to the sequence of PchsA in the Genbank, A specific band was amplified by PCR.And ligated to pMD 18-T vector.PCR size analysis and enzyme digestion indicated that the correct PCR product. Sequengcing and BLAST analysis confirmed the gene was the promoter PchsA gene.F3'5'H gene and PchsA were removed from pMD 18-T vector by PCR,and jointly ligated to plant expression vector pBI 121 and transformed into A. tumefaciens LBA 4404.Transformation system for chrysanthemum transformation was established. The transformation efficiency was enhanced when chrysanthemum was infected with Agrobacterium while its OD value is 0.5 for 7 min, then co-cultured 3 d. On the selective mediums with Kan 50 mg/L, and Carb 500 mg/L, transgenic chrysanthemum calli were obtained.
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